Manipulation of magnetic particles in a biological sample

Patent metadata

Field	Value
Publication number	US-9410948-B2
Application number	US-201013258200-A
Country	US
Kind code	B2
Filing date	Mar 19, 2010
Priority date	Mar 23, 2009
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

A microfluidic device, used with a processing device having a magnetic supplier, including a chamber arranged to receive a biological sample and at least one magnetic particle, and a storage for storing information comprising a magnetic protocol in a form readable by the processing device. The magnetic supplier is configured to generate magnetic forces on the magnetic particle(s) according to this magnetic protocol read from the storage.

First claim

The invention claimed is:

1. A microfluidic device for performing a magnetic particle assay, the microfluidic device comprising:
a chamber configured to receive magnetic particles and a biological sample for mixing with the magnetic particles;
an identifier including information for identifying a magnetic actuation protocol associated with the microfluidic device and conditions of manufacturing of the microfluidic device;
a magnetic supplier configured to generate magnetic forces;
a reader configured to read the information from the identifier; and
a processing device including a processor, a reader and a magnetic supplier,
wherein the reader is configured to read the information from the identifier, the magnetic supplier is configured to generate magnetic forces, and the processor is configured to control the magnetic supplier for generation of magnetic forces on the magnetic particles according to the magnetic actuation protocol identified by the information read from the identifier.

2. The microfluidic device according to claim 1 , wherein the chamber comprises a sensing surface for capturing at least one the magnetic particles and analytes included in the biological sample, wherein the magnetic particles are arranged to specifically bind with the analytes, and wherein the processing device further comprises a detector configured to detect presence of bound magnetic beads bound to the sensing surface.

3. The microfluidic device according to claim 1 , wherein the magnetic particles are attached in the chamber in a dry state so as to be dispersed in the biological sample when the biological sample is received in the chamber.

4. The microfluidic device of claim 1 , wherein the chamber is located proximate the magnetic supplier.

5. The microfluidic device according to claim 1 , wherein the identifier comprises a chip, and when the chamber is placed proximate to or in the processing device, the processing device is configured to automatically read said information from the identifier.

6. The microfluidic device according to claim 1 , wherein the information is in a form selected from at least one of a Radio-Frequency Identification (RFID), a bar code, a 2D bar code, a solid state electronic storage device, an integrated circuit card, and any combination thereof.

7. The microfluidic device according to claim 1 , wherein the identifier comprises a Radio-Frequency Identification (RFID) tag and further comprising an RF emitter configured to send the information to the processing device.

8. The microfluidic device according to claim 7 , wherein the RFID tag is a read/write tag.

9. The microfluidic device according to claim 7 , wherein the RFID tag is a passive or active tag.

10. The microfluidic device according to claim 1 , further comprising at least one magnets, wherein the information further includes at least one of:
at least one frequency, amplitude, duty cycle, polarity and status of each magnet;
position of the magnetic supplier relative to the microfluidic device;
number of repetition cycles of the magnetic forces; and
at least one combination, type and duration of magnetic sequence.

11. A processing device configured to manipulate magnetic particles in a biological sample, the processing device comprising:
a microfluidic device for performing a magnetic particle assay and having
a chamber including magnetic particles and a biological sample for mixing with the magnetic particles, and
an identifier including information for identifying a magnetic protocol associated with the microfluidic device and conditions of manufacturing of the microfluidic device;

a magnetic supplier configured to generate at least one magnetic field according to at least one of a plurality of magnetic protocols so as to manipulate the magnetic particles;
a reader configured to read the identifier when the microfluidic device is within a predetermined distance from the reader; and
a processor configured to control the magnetic supplier according to the magnetic protocol.

12. The processing device according to claim 11 , wherein the reader comprises a receiver to receive the information in a Radio-Frequency (RF) form from the microfluidic device.

13. The processing device of claim 12 , further comprising an RF emitter to send Radio-Frequency Identification (RFID) to the microfluidic device.

14. The processing device according to claim 11 , wherein the information comprises a bar code and the reader comprises an optical bar code reader.

15. A system for manipulating magnetic particles when performing a magnetic particle assay, the system comprising:
a processing device comprising a processor, a reader, and a magnetic supplier configured to generate at least one magnetic field according to at least one of a plurality of magnetic protocols identified by information read by the reader; and
a microfluidic device having
a chamber having one of a plurality of combinations of magnetic particles and a biological sample for mixing with the magnetic particles,
an identifier for identifying a protocol associated with the microfluidic device and conditions of manufacturing type of the microfluidic device, and
a reader configured to read the conditions of manufacturing of the microfluidic device,

wherein the processor is configured to control the magnetic supplier according to the magnetic protocol identified by the information read by the reader.

16. The microfluidic device of claim 1 , wherein the microfluidic device is manufactured in batches and the magnetic actuation protocol is customized for each batch of microfluidic devices, wherein the conditions of manufacturing include a number of microfluidic devices per batch, and wherein the processing device is further configured to delete information regarding the magnetic actuation protocol corresponding to the batch once a last microfluidic device of the batch has been used.

17. The microfluidic device of claim 1 , wherein the processor is further configured to change the magnetic actuation protocol according to a temperature measured when magnetic manipulation takes place.

18. The microfluidic device of claim 1 , wherein the information take into account a reagent contained in the microfluidic device, and wherein the processor is further configured to select among magnetic actuation protocols of the identifier based on a fulfilled condition related to one of a measured temperature and a measured wetting of the microfluidic device.

19. The processing device of claim 11 , wherein the information take into account a reagent contained in the microfluidic device, wherein the microfluidic device is manufactured in batches and the magnetic actuation protocol is customized for each batch of microfluidic devices, wherein the conditions of manufacturing include a number of microfluidic devices per batch, wherein the processor is further configured to delete information regarding the magnetic actuation protocol corresponding to the batch once a last microfluidic device of the batch has been used, wherein the processor is further configured to change the magnetic actuation protocol according to a temperature measured when magnetic manipulation takes place, and wherein the processor is further configured to select among magnetic actuation protocols of the identifier based on a fulfilled condition related to one of a measured temperature and a measured wetting of the microfluidic device.

20. The system of claim 15 , wherein the information take into account a reagent contained in the microfluidic

Assignees

• Dittmer Wendy U
• Koninklijke Philips Nv

Inventors

• Dittmer Wendy U

Classifications

• B01L2400/043

  magnetic forces · CPC title

• G01N27/745

  for detecting magnetic beads used in biochemical assays (concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098) · CPC title

• G01N27/72

  by investigating magnetic variables · CPC title

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• G01N33/54333

  Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction · CPC title