Plant SNF1-related protein kinase gene

Patent metadata

Field	Value
Publication number	US-9410160-B2
Application number	US-75769610-A
Country	US
Kind code	B2
Filing date	Apr 9, 2010
Priority date	Apr 10, 2009
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

The present invention relates to the isolation, purification, characterization and use of the plant Snf1-related protein kinase (SnRK) gene and genetic products. The invention includes isolated and purified SnRK DNA and relates to methods of regulating water loss and plant drought tolerance, sucrose content, starch content, seed oil content, fatty acid synthesis, seed oil acyl composition, seed size/weight, resistance/tolerance to biotic stresses, increased root biomass, and/or carbon flux into other seed components, plant, using the gene, and to tissues and plants transformed with the gene. The invention also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of the invention, and a method of producing such plants and plant seeds.

First claim

What is claimed is:

1. A process for producing a plant, plant seed or progeny thereof, the process comprising:
transforming a plant cell with a heterologous nucleic acid sequence encoding a Snf1-related protein kinase 2-6 protein; wherein the heterologous nucleic acid comprises SEQ ID NO:4;
growing a plant from the plant cell until the plant produces seed;
and harvesting the seed from the plant; wherein the plant is selected from the group consisting of consisting of Arabidopsis thaliana, Borago spp., Canola, Ricinus spp., Theobroma spp., Zea spp., Gossypium spp., Crambe spp., Cuphea spp., Linum spp., Lesquerella spp., Limnanthes spp., Linola, Tropaeolum spp., Oenothera spp., Olea spp., Elaeis spp., Arachis spp., rapeseed, Carthamus spp., Glycine spp., Soja spp., Helianthus spp., Nicotiana spp., Vernonia spp., Triticum spp., Hordeum spp., Oryza spp., Avena spp., Sorghum spp., Secale spp., Brassicaceae, and other members of the plant family Gramineae; and wherein the plant, plant storage organ or plant seed displays enhanced phenotypic traits.

2. The process of claim 1 , wherein the nucleic acid sequence encoding the Snf1-related protein kinase 2-6 protein is integrated into a genome of the plant cell.

3. The process of claim 1 , further comprising selecting the seed having the nucleic acid sequence encoding the Snf1-related protein kinase 2-6 protein integrated into a genome of the plant cell.

4. A seed comprising the heterologous nucleic acid sequence encoding a Snf1-related protein kinase 2-6 protein harvested from the plant produced by the process of claim 1 .

5. The process of claim 1 , further comprising:
planting the seed in soil;
growing a second plant from the seed; and
harvesting the seed from the second plant.

6. The process of claim 1 , wherein transforming the plant cell with the nucleic acid sequence encoding the Snf1-related protein kinase 2-6 protein comprises:
transforming an Agrobacterium cell with the nucleic acid sequence encoding the Snf1-related protein kinase 2-6 protein; and
transforming the plant cell with the Agrobacterium cell.

7. The process of claim 3 , wherein selecting the seed having the nucleic acid sequence encoding the Snf1-related protein kinase 2-6 protein integrated into the genome of the plant cell comprises:
isolating genomic nucleic acid from the seed; and
amplifying the nucleic acid sequence encoding the Snf1-related protein kinase 2-6 protein from the isolated genomic nucleic acid from the seed.

8. The process according to claim 1 , further comprising extracting oil from the harvested seed.

9. A plant, plant seed, or progeny thereof having a heterologous nucleic acid sequence encoding a Snf1-related protein kinase 2-6 protein incorporated in a genome thereof; wherein the heterologous nucleic acid comprises SEQ ID NO:4; and wherein the plant is selected from the group consisting of Arabidopsis thaliana, Borazo spp., Canola, Ricinus spp., Theobroma spp., Zea spp., Gossypium spp., Crambe spp., Cuphea spp., Linum spp., Lesquerella spp., Limnanthes spp., Linola, Tropaeolum spp., Oenothera spp., Olea spp., Elaeis spp., Arachis spp., rapeseed, Carthamus spp., Glycine spp., Sofa spp., Helianthus spp., Nicotiana spp., Vernonia spp., Triticum spp., Hordeum spp., Orvza spp., Avena spp., Sorghum spp., Secale spp., Brassicaceae, and other members of the plant family Gramineae; and wherein the plant, plant storage organ or plant seed displays enhanced phenotypic traits.

10. A method of changing the oil, sugar, or starch content of a plant, plant storage organ or plant seed, said process comprising:
introducing a nucleic acid construct into a plant transformation vector to produce a modified plant transformation vector, wherein said nucleic acid construct comprises a heterologous isolated, purified or recombinant nucleic acid sequence encoding an Arabidopsis Snf1-related protein kinase 2-6 (SnRK2-6) protein; wherein the heterologous isolated, purified or recombinant nucleic acid comprises SEQ ID NO:4;
transforming said plant, plant storage organ or plant seed's genome with said modified plant transformation vector;
and growing said plant, plant storage organ or plant seed and extracting said oil, sugar, or starch; wherein the plant is selected from the group consisting of consisting of Arabidopsis thaliana, Borago spp., Canola, Ricinus spp., Theobroma spp., Zea spp., Gossypium spp., Crambe spp., Cuphea spp., Linum spp., Lesquerella spp., Limnanthes spp., Linola, Tropaeolum spp., Oenothera spp., Olea spp., Elaeis spp., Arachis spp., rapeseed, Carthamus spp., Glycine spp., Soja spp., Helianthus spp., Nicotiana spp., Vernonia spp., Triticum spp., Hordeum spp., Oryza spp., Avena spp., Sorghum spp., Secale spp., Brassicaceae, and other members of the plant family Gramineae; and wherein the plant, plant storage organ or plant seed displays enhanced phenotypic traits.

11. A genetically transformed plant, produced by the method of claim 10 .

12. A genetically transformed plant seed, produced by the method of claim 10 .

13. The genetically transformed plant of claim 11 , wherein the enhanced phenotypic traits is an altered respiration rate as compared to a genomically unmodified plant of the same genotype.

14. The genetically transformed plant of claim 11 , wherein the enhanced phenotypic traits is an altered seed oil content as compared to a genomically unmodified plant of the same genotype.

15. The genetically transformed plant of claim 11 , wherein the enhanced phenotypic traits is an altered fatty acid composition as compared to a genomically unmodified plant of the same genotype.

16. The genetically transformed plant of claim 11 , wherein the enhanced phenotypic traits is an enhanced biomass as compared to a genomically unmodified plant of the same genotype.

17. The genetically transformed plant of claim 11 , wherein the enhanced phenotypic traits is an enhanced capacity to accumulate biopolymers as compared to a genomically unmodified plant of the same genotype.

18. The genetically transformed plant of claim 11 , wherein the enhanced phenotypic traits is an altered carbohydrate content as compared to a genomically unmodified plant of the same genotype.

19. The genetically transformed plant of claim 18 , wherein the enhanced phenotypic traits is an altered sucrose and starch content as compared to a genomically unmodified plant of the same genotype.

20. The genetically transformed plant seed of claim 12 , wherein the enhanced phenotypic traits is an altered respiration rate as compared to a genomically unmodified plant of the same genotype.

21. The genetically transformed plant seed of claim 12 , wherein the enhanced phenotypic traits is an altered seed oil content as compared to a genomically unmodified plant of the same genotype.

22. The genetically transformed plant seed of claim 12 , wherein the seed produces a plant which exhibits an enhanced fatty acid composition compared to a genomically unmodified plant of the same genotype.

23. The genetically transformed plant seed of claim 12 , wherein the seed produces a plant which exhibits an enhanced biomass compared to a genomically unmodified plant of the same genotype.

24. The genetically transformed plant seed of claim 12 , wherein the seed produces a plant which exhibits an enhanced capacity t

Assignees

• Zheng Zhifu
• Greene Thomas W
• Dow Agrosciences Llc

Inventors

• Zheng Zhifu
• Greene Thomas W

Classifications

• C12N15/8245 Primary

  involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title