Thermostable carbonic anhydrases and methods of use thereof

Patent metadata

Field	Value
Publication number	US-9410140-B2
Application number	US-201214113775-A
Country	US
Kind code	B2
Filing date	May 8, 2012
Priority date	May 10, 2011
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide.

First claim

We claim:

1. A composition comprising a recombinant polypeptide and an auxiliary agent, wherein said recombinant polypeptide comprises an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:3, wherein the recombinant polypeptide has carbonic anhydrase activity, and wherein the temperature of the composition is 40° C. to 80° C.

2. The composition of claim 1 , wherein the recombinant polypeptide comprises an amino acid sequence that is at least 98% identical to the amino acid sequence of SEQ ID NO:3.

3. The composition of claim 1 , further comprising carbon dioxide.

4. The composition of claim 3 , wherein the recombinant polypeptide is immobilized.

5. The composition of claim 4 , wherein the recombinant polypeptide is immobilized on a polymer, a membrane, a matrix, a micellar material, a wafer, a solid support, or a micro-particle.

6. A method for extracting carbon dioxide from a carbon dioxide-containing medium, comprising: contacting the carbon dioxide-containing medium with a polypeptide to yield a medium reduced in carbon dioxide, wherein the temperature of the carbon dioxide-containing medium is 40° C. to 80° C., wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:3, and wherein the polypeptide has carbonic anhydrase activity.

7. The method of claim 6 , wherein the carbon dioxide-containing medium is selected from the group consisting of a gas, a liquid, and a multi-phase mixture.

8. The method of claim 6 , wherein the carbon dioxide-containing medium is a gas selected from the group consisting of a flue gas, a raw natural gas, a syngas, a hydrogen gas, and a biogas.

9. The method of claim 6 , wherein the carbon dioxide-containing medium is a by-product of combustion or fermentation.

10. A method for regulating pH of a medium containing one or both of CO 2 and bicarbonate, comprising: contacting the medium with a polypeptide of to yield a medium with an altered pH, wherein the temperature of the medium is 40° C. to 80° C., wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:3, and wherein the polypeptide has carbonic anhydrase activity.

Assignees

• Ge Jing
• Hua Ling
• Poulose Ayrookaran J
• Danisco Us Inc

Inventors

• Ge Jing
• Hua Ling
• Poulose Ayrookaran J

Classifications

• C12Y402/01001

  Carbonate dehydratase (4.2.1.1), i.e. carbonic anhydrase · CPC title

• C02F3/342

  characterised by the enzymes used · CPC title

• Y02E50/343

  Cross-Sectional Technologies · mapped topic

• C12N9/88 Primary

  Lyases (4.) · CPC title

• B01D2251/95

  Specific microorganisms · CPC title