Antibody composition-producing cell

Patent metadata

Field	Value
Publication number	US-9409982-B2
Application number	US-201414520528-A
Country	US
Kind code	B2
Filing date	Oct 22, 2014
Priority date	Oct 6, 2000
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

The present invention relates to a cell for the production of an antibody molecule such as an antibody useful for various diseases having high antibody-dependant cell-mediated cytotoxic activity, a fragment of the antibody and a fusion protein having the Fc region of the antibody or the like, a method for producing an antibody composition using the cell, the antibody composition and use thereof.

First claim

What is claimed is:

1. An animal cell containing a DNA encoding an antibody, wherein said animal cell is modified, by a modification, to exhibit a reduced amount, or an absence, of N-glycoside-linked sugar chains containing fucose linked through an α-1,6 linkage, and wherein said animal cell produces antibodies that do not contain fucose linked through an α-1,6 linkage,
wherein said modification causes a reduction in, or an absence of, at least one intracellular sugar nucleotide in said cell, said at least one intracellular sugar nucleotide being selected from the group consisting of: GDP-α4-keto-6-deoxymannose; GDP-α4-keto-6-deoxygalactose; and GDP-fucose.

2. The animal cell according to claim 1 , wherein said modification is the introduction into said cell, or the deletion from said cell, of an enzyme, said introduction or deletion causing a reduction in the amount, or an absence, of the at least one intracellular sugar nucleotide in said cell.

3. The animal cell according to claim 2 , wherein the enzyme is an enzyme which alters the structure of a substrate for an enzyme in a GDP-fucose synthesis pathway.

4. The animal cell according to claim 3 , wherein the enzyme in the GDP-fucose synthesis pathway is GDP-keto-6-deoxymannose 3,5-epimerase 4-reductase.

5. The animal cell according to claim 1 , wherein the antibodies produced by said cell that do not contain fucose linked through an α-1,6 linkage constitute 20% or more of the total antibodies produced by said cell.

6. The animal cell according to claim 5 , wherein the antibodies produced by said cell that do not contain fucose linked through an α-1,6 linkage constitute 50% or more of the total antibodies produced by said cell.

7. The animal cell according to claim 1 , wherein the animal cell is a CHO cell.

Assignees

• Kyowa Hakko Kirin Co Ltd

Inventors

• Kanda Yutaka
• Satoh Mitsuo
• Nakamura Kazuyasu
• Uchida Kazuhisa
• Shinkawa Toyohide
• Yamane Naoko
• Hosaka Emi
• Yamano Kazuya
• Yamasaki Motoo
• Hanai Nobuo

Classifications

• A61P37/02

  Immunomodulators · CPC title

• A61P37/00

  Drugs for immunological or allergic disorders · CPC title

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61P31/12

  Antivirals · CPC title