Methods for detecting inflammatory bowel disease in a feline

Patent metadata

Field	Value
Publication number	US-9408817-B2
Application number	US-201213372251-A
Country	US
Kind code	B2
Filing date	Feb 13, 2012
Priority date	Dec 29, 2005
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

Compositions comprising docosahexaenoic acid (DHA) and optionally one or more fatty acids selected from the group consisting of eicosapentaenoic acid (EPA), arachidonic acid (ARA), linoleic acid (LA), and α-linoleic acid (ALA) are administered to felines for preventing or treating feline inflammatory bowel disease (IBD).

First claim

What is claimed is:

1. A method for determining if a feline is suffering from inflammatory bowel disease comprising:
collecting lymphocytes from blood obtained from the feline as a first sample;
collecting lymphocytes from blood obtained from a healthy feline as a second sample;
exposing the second sample to an amount of a mitogen for a period of time;
exposing the first sample to the same amount of the same mitogen as the second sample for the same period of time as the second sample in the presence of from about 12.5 μM to about 25 μM docosahexaenoic acid or eicosapentaenoic acid;
measuring the gene expression of one or more of the cytokines selected from the group consisting of interleukin-2 (IL-2) and interferon gamma (IFN-γ); and
comparing levels of gene expression in all samples, wherein if the level of gene expression in the second sample is similar to the level of gene expression in the first sample, then the feline is suffering from inflammatory bowel disease.

2. The method of claim 1 , wherein the mitogen is selected from the group consisting of phytohemagglutinin, concanavalin, pokeweed mitogen, lipopolysaccharide, and anti-CD3 antibody.

Assignees

• Khoo Christina
• Schoenherr William David
• Gross Kathy Lynn
• Hills Pet Nutrition Inc

Inventors

• Khoo Christina
• Schoenherr William David
• Gross Kathy Lynn

Classifications

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61K9/0056

  Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals · CPC title

• A61P1/00

  Drugs for disorders of the alimentary tract or the digestive system · CPC title

• A61P1/04

  for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants · CPC title

• A61K31/202 Primary

  having three or more double bonds, e.g. linolenic (eicosanoids, e.g. leukotrienes A61K31/557) · CPC title