Method for preparing an outer surface of a planar waveguide to be capable of binding target samples along a plurality of predeterminded lines and a planar waveguide

Patent metadata

Field	Value
Publication number	US-9405069-B2
Application number	US-201414655923-A
Country	US
Kind code	B2
Filing date	Jan 17, 2014
Priority date	Jan 17, 2013
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

According to the invention there is provided a method for preparing the outer surface ( 11 ) of the planar waveguide ( 1 ) for binding target samples along the plurality of predetermined lines ( 4 ). The method comprises the following steps. Providing a planar waveguide ( 1 ) with an outer surface ( 11 ) adapted for attachment of a head group ( 21 ) of a linker molecule ( 2 ) to the outer surface ( 11 ). Sequentially applying at least one plurality of linker molecules ( 2, 5 ) to the outer surface ( 11 ). Each plurality of the at least one plurality of linker molecules ( 2, 5 ) assembles to form an individual layer of linker molecules ( 2, 5 ) with individual layers formed one above the other starting from the outer surface ( 11 ) of the planar waveguide ( 1 ). Each linker molecule ( 2, 5 ) comprises a functional group ( 22, 52 ) and the head group ( 21, 51 ) which is capable of attaching to the outer surface ( 11 ) of the planar waveguide ( 1 ) or to the functional groups ( 22 ) of the preceding layer of linker molecules ( 2 ). The functional groups ( 52 ) of the plurality of linker molecules ( 5; 2 ) of the uppermost layer are bound to photo-labile protecting groups ( 3 ) so that each functional group ( 52 ) bound to a said photo-labile protecting group ( 3 ) is incapable of attaching a complementary functional group of a further molecule. Exposing those photo-labile protecting groups ( 3 ) of the uppermost layer arranged along a plurality of predetermined lines ( 4 ) to light of a predetermined wavelength to remove the exposed photo-labile protecting groups ( 3 ) from the functional groups ( 52 ) to make these functional groups ( 52 ) capable of attaching a complementary functional group of a further molecule.

First claim

The invention claimed is:

1. A method for preparing an outer surface of a planar waveguide so as to be capable of binding target samples along a plurality of predetermined lines, the method comprising the steps of:
providing a planar waveguide having an outer surface adapted for attachment of a linker molecule to the outer surface;
sequentially applying at least one plurality of linker molecules to the outer surface, each plurality of the at least one plurality of linker molecules assembling to form an individual layer of linker molecules with individual layers being formed one above the other starting from the outer surface of the planar waveguide, each linker molecule comprising a functional group and a head group, the head group being capable of attaching to the outer surface of the planar waveguide or to the functional groups of the preceding layer of linker molecules, and wherein the functional groups of the linker molecules of the uppermost layer are bound to photo-labile protecting groups so that each functional group of the uppermost layer bound to a said photo-labile protecting group is incapable of attaching a complementary functional group of a further molecule; and
exposing those photo-labile protecting groups of the uppermost layer arranged along a plurality of predetermined lines to light of a predetermined wavelength to remove the exposed photo-labile protecting groups from the functional groups to make these functional groups capable of attaching a complementary functional group of a further molecule.

2. A method according to claim 1 , wherein the step of sequentially applying the at least one plurality of linker molecules consists of applying only two pluralities of linker molecules, the two pluralities of linker molecules assembling to form two individual layers of linker molecules one above the other starting from the outer surface of the planar waveguide.

3. A method according to claim 1 , further comprising the steps of:
attaching complementary functional groups of capture molecules to the functional groups of the uppermost layer of linker molecules arranged along the plurality of predetermined lines, each capture molecule being capable of binding a target sample;
exposing those photo-labile protecting groups arranged between the plurality of predetermined lines to light of the predetermined wavelength to remove the exposed photo-labile protecting groups from the functional groups to make these functional groups capable of attaching a complementary functional group of a said further molecule; and
attaching complementary functional groups of modified capture molecules to the functional groups arranged between the plurality of predetermined lines, each modified capture molecule being incapable of binding to that target sample to which the capture molecules are capable of binding.

4. A method according to claim 3 , wherein the step of exposing those photo-labile protecting groups arranged along the plurality of predetermined lines is carried out for a first exposure dose, and wherein the step of exposing those photo-labile protecting groups arranged between the plurality of predetermined lines is carried out for a second exposure dose, the first exposure dose being different from the second exposure dose.

5. A method according to claim 4 , wherein the first exposure dose and the second exposure dose are less than 10 Joules/cm 2 .

6. A method according to claim 1 , wherein the step of sequentially applying the at least one plurality of linker molecules consists of applying only one plurality of linker molecules.

7. A method according to claim 1 , further comprising the step of attaching head groups of a further plurality of linker molecules to the functional groups of the preceding layer of linker molecules which are arranged along the plurality of predetermined lines, the functional groups of the linker molecules of the further plurality of linker molecules being unbound to photo-labile protecting groups and being capable of attaching a complementary functional group of a further molecule.

8. A method according to claim 7 , further comprising the steps of:
attaching complementary functional groups of capture molecules to the functional groups of linker molecules of the further plurality of linker molecules arranged along the plurality of predetermined lines;
exposing those photo-labile protecting groups arranged between the plurality of predetermined lines to light of the predetermined wavelength to remove the exposed photo-labile protecting groups from the functional groups to make these functional groups capable of attaching a complementary functional group of a further molecule;
attaching complementary functional groups of another further plurality of linker molecules to the functional groups of the preceding layer of linker molecules which are arranged between the plurality of predetermined lines, the functional groups of the linker molecules of the another further plurality of linker molecules being unbound to photo-labile protecting groups and being capable of attaching a complementary functional group of a said further molecule; and
attaching complementary functional groups of modified capture molecules to the functional groups of linker molecules of the further plurality of linker molecules arranged between the plurality of predetermined lines.

9. A method according to claim 1 , wherein the step of exposing those photo-labile protecting groups arranged along the plurality of predetermined lines comprises the steps of:
disposing a phase mask in the proximity of the photo-labile protecting groups, the phase mask having a plurality of protrusions having a first refractive index n 1 , the first refractive index n 1 being different from a second refractive index n 2 of a medium arranged between the phase mask and the outer surface of the planar waveguide; and
transmitting light of the predetermined wavelength through the phase mask for exposing those photo-labile protecting groups arranged along the plurality of predetermined lines.

10. A method according to claim 1 , wherein the plurality of linker molecules of which the head groups attach to the outer surface assemble to form a individual layer having a thickness of 0.5 nm to 10 nm.

11. A method according to claim 1 , wherein the plurality of linker molecules of which the head groups attach to the outer surface assemble to form a monolayer of linker molecules.

12. A method according to claim 1 , wherein the plurality of linker molecules of which the head groups attach to the functional groups of the preceding layer of linker molecules assemble to form the uppermost individual layer having a thickness of 10 nm to 200 nm.

13. A method according to claim 1 , wherein the plurality of predetermined lines are arranged in at least two separate diffraction spots at the outer surface of the planar waveguide, each diffraction spot has an area larger than 25 μm 2 and wherein the plurality of predetermined lines has a distance between adjacent lines less than 1.5 μm, in particular less than 1 μm.

14. A planar waveguide having an outer surface prepared to be capable of binding target samples along a plurality of predetermined lines, wherein the plurality of predetermined lines are arranged in at least two separate diffraction spots at the outer surface, each diffraction spot has an area larger than 25 μm 2 , and wherein the plurality of predetermined lines has a distance between adjacent lines less than 1.5 μm, in particular less than 1 μm.

15. A planar waveguide according to claim 14 , wherein the plurality of predetermined lines are arranged with respect

Assignees

• Hoffmann La Roche

Inventors

• Fattinger Christof

Classifications

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title

• G02B6/26 Primary

  Optical coupling means (G02B6/36, G02B6/42 take precedence) · CPC title

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• G01N33/543 Primary

  with an insoluble carrier for immobilising immunochemicals · CPC title