Glucose dehydrogenase

Patent metadata

Field	Value
Publication number	US-9404144-B2
Application number	US-201314374164-A
Country	US
Kind code	B2
Filing date	Feb 7, 2013
Priority date	Feb 9, 2012
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.

First claim

The invention claimed is:

1. A glucose assay kit, comprising:
a flavin-binding glucose dehydrogenase, the flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity;
and a mediator.

2. A glucose sensor, comprising:
a flavin-binding glucose dehydrogenase immobilized on an electrode, the flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having glucose dehydrogenase activity.

3. The glucose assay kit according to claim 1 , further comprising a buffer solution.

4. The glucose sensor according to claim 2 , wherein the electrode is selected from one of the following electrodes:
carbon electrode, platinum electrode and gold electrode.

5. The glucose assay kit according to claim 1 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 85% or more identity to the amino acid sequence of SEQ ID NO: 1.

6. The glucose assay kit according to claim 1 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 90% or more identity to the amino acid sequence of SEQ ID NO: 1.

7. The glucose assay kit according to claim 1 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 95% or more identity to the amino acid sequence of SEQ ID NO: 1.

8. The glucose assay kit according to claim 1 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 98% or more identity to the amino acid sequence of SEQ ID NO: 1.

9. The glucose assay kit according to claim 1 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 99% or more identity to the amino acid sequence of SEQ ID NO: 1.

10. The glucose sensor according to claim 2 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 85% or more identity to the amino acid sequence of SEQ ID NO: 1.

11. The glucose sensor according to claim 2 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 90% or more identity to the amino acid sequence of SEQ ID NO: 1.

12. The glucose sensor according to claim 2 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 95% or more identity to the amino acid sequence of SEQ ID NO: 1.

13. The glucose sensor according to claim 2 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 98% or more identity to the amino acid sequence of SEQ ID NO: 1.

14. The glucose sensor according to claim 2 , wherein the polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1 is a polypeptide having an amino acid sequence with 99% or more identity to the amino acid sequence of SEQ ID NO: 1.

Assignees

• Toyo Boseki

Inventors

• Sumida Yosuke
• Hirao Rie
• Utashima Yuu
• Kawaminami Hiroshi
• Aiba Hiroshi
• Kishimoto Takahide
• Yanagidani Shusaku

Classifications

• C12Y101/05

  with a quinone or similar compound as acceptor (1.1.5) · CPC title

• G01N2333/904

  acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1) · CPC title

• C12N9/0006

  acting on CH-OH groups as donors (1.1) · CPC title

• C12Q1/32 Primary

  involving dehydrogenase · CPC title

• C12Y101/01047

  Glucose 1-dehydrogenase (1.1.1.47) · CPC title