Thermophilic and thermoacidophilic biopolymer degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods

Patent metadata

Field	Value
Publication number	US-9404134-B2
Application number	US-201514727653-A
Country	US
Kind code	B2
Filing date	Jun 1, 2015
Priority date	Jan 31, 2008
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

First claim

What is claimed is:

1. A method of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups, the method comprising:
placing a polypeptide at least 90% sequence identity to SEQ ID No. 304 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group;
wherein the polypeptide has an enzymatic activity as an carboxylesterase.

2. The method according to claim 1 , wherein placing a polypeptide at least 90% sequence identity to SEQ ID No. 304 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group occurs at or below about pH 4.

3. The method according to claim 1 , wherein placing a polypeptide having at least 90% sequence identity to SEQ ID No. 304 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group occurs at a temperature at or above 50 degrees Celsius.

4. The method according to claim 1 , wherein the polypeptide is glycosylated, pegylated, or otherwise posttranslationally modified.

5. The method according to claim 1 , wherein the polypeptide is encoded by a nucleic acid having at least 90% identity to SEQ ID NO:303.

6. The method according to claim 1 , wherein placing a polypeptide having at least 90% sequence identity to SEQ ID NO:304 in fluid contact with a polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group comprises translating a nucleic acid having at least 90% identity to SEQ ID NO:303 in fluid contact with the polysaccharide, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycoside, xylan-, glucan-, galactan-, or mannan-decorating group.

Assignees

• Battelle Energy Alliance Llc

Inventors

• Thompson David N
• Apel William A
• Thompson Vicki S
• Reed David W
• Lacey Jeffrey A
• Henriksen Emily D

Classifications

• C12N9/88

  Lyases (4.) · CPC title

• C12N9/2482

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• C12Y302/01091

  Cellulose 1,4-beta-cellobiosidase (3.2.1.91) · CPC title

• C12N9/2445

  Beta-glucosidase (3.2.1.21) · CPC title

• C12Y301/02004

  3-Hydroxyisobutyryl-CoA hydrolase (3.1.2.4) · CPC title