Peptide capable of silica deposition and use thereof

Patent metadata

Field	Value
Publication number	US-9404097-B2
Application number	US-201214348322-A
Country	US
Kind code	B2
Filing date	Oct 2, 2012
Priority date	Sep 30, 2011
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

A peptide for synthesizing silica and use thereof are provided. The peptide for synthesizing silica can polymerize silica from a silica precursor in an aqueous solution having conditions of normal temperature, normal pressure and near-neutral weak base. The peptide for synthesizing silica can form a self-assembled structure during silica synthesis, and thus can be used as various biomaterials such as a silica-based protein immobilizer, a biosensor, and a drug delivery system.

First claim

What is claimed is:

1. A method of synthesizing silica, comprising:
reacting a silica precursor with at least one peptide, wherein said peptide is selected from the group consisting of amino acid sequences set forth in SEQ ID NOs: 1 to 7, and a fusion protein thereof, wherein said fusion protein comprises a self-assembling protein and at least one peptide selected from the group consisting of amino acid sequences set forth in SEQ ID Nos: 1 to 7.

2. The method of claim 1 , wherein the reacting is performed in an aqueous solution of pH 6 to 8 under conditions of normal temperature and a normal pressure.

3. The method of claim 1 , wherein the reacting is performed in the aqueous solution further comprising an enzyme or a binding peptide, wherein the binding peptide is at least one selected from the group consisting of an antibody, a peptide specifically binding to DNA or RNA, or a receptor.

4. The method of claim 1 , wherein the reacting is performed in the aqueous solution further comprising a nanotube or a nanomesh.

5. The method of claim 1 , wherein the reacting is performed in the aqueous solution further comprising a phospholipid.

6. The method of claim 1 or 5 , wherein the reacting is performed for preparing a single silica film by allowing at least one peptide selected from the group consisting of amino acid sequences set forth in SEQ ID NOs: 1 to 7, a silica precursor and a phospholipid to react with each other, or synthesizing a porous silica by allowing the peptide, the silica precursor and the phospholipid to react with each other to prepare an organic/inorganic complex, and treating the organic/inorganic complex with heat or an organic solvent to remove an organic substance.

7. The method of claim 1 , wherein the reacting is performed in the aqueous solution further comprising hydroxyapatite.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Pack Seung Pil
• Ki Mi Ran

Classifications

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• A61K49/00

  Preparations for testing in vivo · CPC title

• C12Y207/01

  Phosphotransferases with an alcohol group as acceptor (2.7.1) · CPC title

• C12N9/1205 Primary

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

• C07K17/14

  Peptides being immobilised on, or in, an inorganic carrier · CPC title