Peptides useful as cell-penetrating peptides

Patent metadata

Field	Value
Publication number	US-9403884-B2
Application number	US-201213557706-A
Country	US
Kind code	B2
Filing date	Jul 25, 2012
Priority date	Dec 30, 2005
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

A peptide having an amino acid sequence containing at least eight consecutive amino acids of the human lactoferrin protein or of the bovine lactoferrin protein. The peptide is suitable as a cell-penetrating peptide. A complex of the peptide and a cargo molecule non-covalently bound to the peptide. The cargo molecule may be a nucleic acid, an amino acid, a peptide, a protein, a carbohydrate, a lipid, or a small molecule. A method of penetrating or transfecting a cell using the complex.

First claim

The invention claimed is:

1. A complex, comprising:
(A) a peptide consisting of SEQ ID NO:3 and which is not-labeled with a radioactive label or a hapten; and
(B) a cargo molecule non-covalently bound to the peptide, wherein the non-covalent bond is an ionic bond, a hydrogen bond, or a hydrophobic interaction.

2. The complex of claim 1 , wherein the cargo molecule is at least one selected from the group consisting of a nucleic acid, an amino acid, a peptide, a protein, a carbohydrate, a lipid, and a small molecule.

3. The complex of claim 1 , wherein the cargo molecule is present as or part of a structure,
wherein the structure is selected from the group consisting of nanoparticles, microparticles, liposomes, and micelles.

4. The complex of claim 2 , wherein the nucleic acid is a nucleic acid selected from the group consisting of DNA molecules, RNA molecules, PNA molecules, siRNA molecules, antisense molecules, ribozymes, aptamers, spiegelmers, and decoy molecules.

5. The complex of claim 2 , wherein the cargo molecule is a peptide intended for use as a vaccine.

6. The complex of claim 2 , wherein the nucleic acid is a nucleic acid-based vaccine.

7. The complex of claim 3 , wherein the structure is at least one of the nanoparticles and the microparticles and the at least one of the nanoparticles and the microparticles comprise a pharmaceutically active compound.

8. The complex of claim 7 , wherein at least one of the nanoparticles and the microparticles consist of a pharmaceutically active compound.

9. The complex of claim 1 ,
wherein the cargo molecule is a small molecule having a molecular weight of 1000 D or less and is a drug or a drug candidate non-covalently bound to the peptide.

10. A complex, consisting of:
(A) a peptide consisting of SEQ ID NO:3; and
(B) a cargo molecule non-covalently bound to the peptide, wherein the non-covalent bond is an ionic bond, a hydrogen bond, or a hydrophobic interaction.

Assignees

• Brock Roland
• Fischer Rainer
• Fotin-Mleczek Mariola
• Hufnagel Hansjoerg
• Windhab Norbert
• Evonik Roehm Gmbh

Inventors

• Brock Roland
• Fischer Rainer
• Fotin-Mleczek Mariola
• Hufnagel Hansjoerg
• Windhab Norbert

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• A61P31/10

  Antimycotics · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61P17/06

  Antipsoriatics · CPC title

• A61P19/02

  for joint disorders, e.g. arthritis, arthrosis · CPC title