Peptides and use thereof

Patent metadata

Field	Value
Publication number	US-9403874-B2
Application number	US-201314412020-A
Country	US
Kind code	B2
Filing date	Jul 3, 2013
Priority date	Jul 3, 2012
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

The present invention relates to novel peptides and use thereof and more specifically is directed to a peptide with anti-inflammatory effect, a polynucleotide encoding the peptide, a pharmaceutical composition comprising the peptide or polynucleotide for preventing or treating inflammatory diseases, an anti-inflammatory drug, an over-the-counter (OTC) drug composition comprising the peptide for preventing or ameliorating inflammation, a health food composition for alleviating or ameliorating inflammation, a cosmetic composition for preventing or ameliorating inflammation, a method for treating inflammatory diseases, comprising administrating the pharmaceutical composition to the subject suspected of having inflammatory disease, a method for preparing a mimetic of the peptide and a method for designing the same.

First claim

The invention claimed is:

1. An isolated peptide represented by an amino acid sequence of SEQ ID NO: 1 or a fragment thereof.

2. The isolated peptide or fragment thereof according to claim 1 , wherein the fragment comprises:
a. 6 to 13 consecutive amino acid residues, comprising the 4 th to 6 th amino acids (proline-serine-proline, PSP) of an amino acid sequence of SEQ ID NO: 1;
b. 4 to 10 consecutive amino acid residues, comprising the 10 th to 13 th amino acids (arginine-threonine-aspartic acid-glycine, RTDG) of an amino acid sequence of SEQ ID NO: 1;
c. 6 to 10 consecutive amino acid residues, comprising the 14 th to 16 th amino acids (arginine-threonine-aspartic acid, RTD) of an amino acid sequence of SEQ ID NO: 1;
d. 2 to 6 consecutive amino acid residues, comprising the 12 th and 13 th amino acids (aspartic acid-glycine, DG) of an amino acid sequence of SEQ ID NO: 1; or
e. 2 to 6 consecutive amino acid residues, comprising the 7 th and 8 th amino acids (arginine-aspartic acid, RD) of an amino acid sequence of SEQ ID NO: 1.

3. The isolated peptide or fragment thereof according to claim 2 , wherein the fragment is any one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 2 to 18.

4. The isolated peptide or fragment thereof according to claim 1 , wherein the peptide or fragment thereof has an anti-inflammatory effect.

5. A polynucleotide encoding the peptide or fragment thereof of claim 1 .

6. A pharmaceutical composition for preventing or treating inflammatory disease, comprising the peptide or fragment thereof of claim 1 .

7. The composition according to claim 6 , which further comprises a pharmaceutically acceptable carrier.

8. The composition according to claim 6 , wherein the inflammatory disease is selected from the group consisting of atopy, psoriasis, dermatitis, allergy, arthritis, rhinitis, otitis media, pharyngolaryngitis, tonsillitis, cystitis, nephritis, pelvic inflammatory disease, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematodes (SLE), atherosclerosis, asthma, arteriosclerosis, edema, rheumatoid arthritis, delayed allergy (IV-type allergy), graft rejection, graft-versus-host disease, autoimmune encephalomyelitis, multiple sclerosis, inflammatory bowel disease, cystic fibrosis, diabetic retinopathy, ischemia-reperfusion injury, restenosis, glomerulonephritis and gastrointestinal allergy.

9. An anti-inflammatory drug, comprising the peptide or fragment thereof of claim 1 .

10. An over-the-counter (OTC) drug composition for preventing or ameliorating inflammation, comprising the peptide or fragment thereof of claim 1 .

11. A health food composition for alleviating or ameliorating inflammation, comprising the peptide or fragment thereof of claim 1 .

12. A cosmetic composition for preventing or alleviating inflammation, comprising the peptide or fragment thereof of claim 1 .

13. A method for treating inflammatory disease, comprising administrating the composition of claim 6 to a subject suspected of having inflammatory disease.

14. A method for preparing a mimetic of the peptide of claim 1 , comprising designing a mimetic of peptide with an anti-inflammatory activity based on the peptide or fragment thereof of claim 1 ; and
a. synthesizing the designed mimetic of peptide.

15. A method for designing a mimetic of the peptide with an anti-inflammatory activity from the peptide or fragment thereof of claim 1 .

Assignees

• Il Yang Pharm Co Ltd
• Industry-Academic Coop Found Sookmyung Women'S Univ
• Samsung Life Public Welfare Foundation
• Il Yang Pharm Co Ltd

Inventors

• Cho Dae Ho
• Jung Min Kyung
• Ha Soo Gyeong
• Park Jeong Min
• Lee Jin Young
• Kim Sang Yoon
• Park Seung Beom
• Kim Hee Jong
• Ju Hyun Soo
• Lee Si Nae
• Lim Hae Kyoung
• Bang Sa Ik

Classifications

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P7/10

  Antioedematous agents; Diuretics · CPC title

• A61P37/06

  Immunosuppressants, e.g. drugs for graft rejection · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title

• A61P25/08

  Antiepileptics; Anticonvulsants · CPC title