Methods of phosvitin extraction and phosphopeptide preparation from egg yolk

Patent metadata

Field	Value
Publication number	US-9403870-B2
Application number	US-201214123890-A
Country	US
Kind code	B2
Filing date	Jun 11, 2012
Priority date	Jun 10, 2011
Publication date	Aug 2, 2016
Grant date	Aug 2, 2016

Abstract

The invention is directed to methods for extracting phosvitin from egg yolk involving contacting the egg yolk or egg yolk protein granules with a solution having a salt concentration of about 10% to form a mixture; optionally, heating the mixture; adjusting the pH of the mixture to separate phosvitin from other proteins; recovering the phosvitin. The phosvitin extract may be dephosphorylated and hydrolyzed to produce phosvitin phosphopeptides.

First claim

What is claimed is:

1. A process for extracting phosvitin from egg yolk comprising the steps of:
a) contacting the egg yolk or egg yolk protein granules with a salt solution to form a mixture comprising phosvitin and non-phosvitin proteins in solution;
b) adjusting the pH of the mixture to 7.0 or below and above about 3.5 to precipitate non-phosvitin proteins and leave phosvitin substantially in solution;
c) separating precipitated proteins; and
d) recovering a solution or supernatant comprising phosvitin.

2. The process of claim 1 , wherein the egg yolk is diluted and mixed with water to produce egg yolk protein granules.

3. The process of claim 1 , wherein pH of the mixture is about 7.

4. The process of claim 1 , wherein the egg yolk or egg yolk protein granules are mixed with the salt solution at a temperature of about 4° C.

5. The process of claim 1 , wherein the egg yolk is mixed with the salt solution and heated.

6. The process of claim 5 wherein the mixture is heated to about 80° C.

7. The process of claim 1 , wherein the salt solution is a 10% sodium chloride solution.

8. The process of claim 1 , wherein the pH is adjusted to a pH of about 5.0 or lower.

9. The process of claim 1 , wherein in step (c) the mixture is centrifuged to remove precipitated components.

10. A process of preparing phosvitin phosphopeptides, comprising the steps of extracting phosvitin from egg yolk using the method of claim 1 , dephosphorylating the phosvitin and hydrolyzing the dephosphorylated phosvitin.

11. The process of claim 10 wherein the phosvitin is dephosphorylated with an alkaline solution.

12. The process of claim 11 wherein the alkaline solution comprises NaOH.

13. The process of claim 10 , wherein the phosvitin is hydrolyzed with a protease.

14. The process of claim 13 wherein the protease comprises pancreatin.

15. The process of claim 14 , wherein hydrolysis is conducted using pancreatin at an enzyme/substrate ratio of about 1:50 (w/w).

16. The process of claim 14 wherein the phosvitin is hydrolyzed to a degree of hydrolysis of greater than about 6%, or greater than about 10%.

17. The process of claim 16 wherein the degree of hydrolysis is about 12.9%.

18. The process of claim 14 wherein the hydrolysate has a nitrogen to phosphorus (N/P) of less than about 5, or less than about 4.

19. The process of claim 10 , which does not use an organic solvent or a non-food compatible chemical.

20. Phosvitin extract obtained by the process of claim 1 , having a purity in a range of about 50% to about 95%.

21. The phosvitin extract of claim 20 having a purity of greater than about 75%, or greater than about 80%.

22. A phosphopeptide derived from the phosvitin extract of claim 20 .

23. A pharmaceutical or nutraceutical composition, or a cosmetic or dental product, or a foodstuff, comprising a phosphopeptide of claim 22 , or both.

24. A method of promoting health by administering to a subject the phosphopeptide of claim 22 , in an amount sufficient to effect a health benefit.

Assignees

• Wu Jianping
• Lei Bo
• Ren Jiandong
• Univ Alberta

Inventors

• Wu Jianping
• Lei Bo
• Ren Jiandong

Classifications

• A61P3/02

  Nutrients, e.g. vitamins, minerals · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title

• C07K7/06 Primary

  having 5 to 11 amino acids · CPC title

• A61K8/981

  of mammals or bird · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title