Alkyl amines improve detection of components of formaldehyde-fixed biological samples

Patent metadata

Field	Value
Publication number	US-9400235-B2
Application number	US-201314139837-A
Country	US
Kind code	B2
Filing date	Dec 23, 2013
Priority date	Mar 30, 2007
Publication date	Jul 26, 2016
Grant date	Jul 26, 2016

Abstract

Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

First claim

What is claimed is:

1. A reaction mixture comprising,
formaldehyde cross-linked biological sample; and
a sufficient amount of an alkyl amine selected from the group consisting of ethylenediamine and propylamine to release at least a portion of a cross-linked component of the formaldehyde cross-linked biological sample.

2. The reaction mixture of claim 1 , wherein the amount of alkyl amine is between 0.01% and 5%.

3. The reaction mixture of claim 1 , wherein the alkyl amine is ethylenediamine.

4. The reaction mixture of claim 1 , wherein the alkyl amine is propylamine.

5. The reaction mixture of claim 1 , wherein the biological sample is a tissue sample from an animal.

6. The reaction mixture of claim 1 , comprising a protease.

7. The reaction mixture of claim 6 , wherein the protease is proteinase K.

Assignees

• Roche Molecular Systems Inc

Inventors

• Will Stephen G
• Bodepudi Veeraiah
• Fiss Ellen H
• Shahinian Rachel

Classifications

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• G01N1/30 Primary

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• C12Q2523/101

  Crosslinking agents, e.g. psoralen · CPC title