Microorganisms of Corynebacterium which can utilize xylose and method for producing L-lysine using same

Patent metadata

Field	Value
Publication number	US-9399784-B2
Application number	US-201514923825-A
Country	US
Kind code	B2
Filing date	Oct 27, 2015
Priority date	Jan 10, 2012
Publication date	Jul 26, 2016
Grant date	Jul 26, 2016

Abstract

The present invention relates to microorganisms of corynebacterium which can utilize xylose and to a method for producing L-lysine using same. More particularly, the present invention relates to microorganisms of corynebacterium which are modified, in which genes encoding xylose isomerase and xylulokinase which are xylose synthases are introduced to express the xylose synthase. The present invention also relates to a method for producing L-lysine, comprising a step of culturing the modified microorganisms of corynebacterium using xylose as a carbon source, and recovering L-lysine from the culture.

First claim

What is claimed is:

1. A Corynebacterium sp. microorganism, wherein said microorganism is transformed with a polynucleotide encoding an Erwinia carotovora xylose isomerase (XylA) and a polynucleotide encoding an Erwinia carotovora xylulokinase (XylB), and wherein the microorganism expresses the E. carotovora XylA and E. carotovora XylB and utilizes xylose when present in a culture medium to produce L-lysine.

2. The microorganism according to claim 1 , wherein the E. carotovora XylA comprises the amino acid sequence of SEQ ID NO: 1, and the E. carotovora XylB comprises the amino acid sequence of SEQ ID NO: 2.

3. The microorganism according to claim 1 , wherein the E. carotovora XylA is encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 3, and the E. carotovora XylB is encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 4.

4. The microorganism according to claim 1 , wherein:
the polynucleotides encoding the E. carotovora XylA and E. carotovora XylB are integrated into the chromosome of the microorganism,
the polynucleotides encoding the E. carotovora XylA and E. carotovora XylB are present within a vector,
the polynucleotides encoding the E. carotovora XylA and E. carotovora XylB are operably linked to a promoter that is heterologous to the polynucleotides, or
a combination thereof.

5. A method for producing L-lysine, comprising the steps of:
(i) culturing the Corynebacterium sp. microorganism of claim 1 in a culture medium containing xylose as a carbon source so as to obtain a culture broth comprising L-lysine; and
(ii) recovering the L-lysine from the culture broth.

6. The method according to claim 5 , wherein the E. carotovora XylA comprises the amino acid sequence of SEQ ID NO: 1, and the E. carotovora XylB comprises the amino acid sequence of SEQ ID NO: 2.

7. The microorganism according to claim 4 , wherein the promoter comprises the nucleotide sequence of SEQ ID NO: 14.

Assignees

• Cj Cheiljedang Corp

Inventors

• Rah So Yeon
• Huh Lan
• Kim Chang Gyeom
• Lee Kwang Ho
• Moon Jun Ok
• Lee Kyung Han
• Sung Jin Seok
• Kim Hyung Joon

Classifications

• C12N9/92

  Glucose isomerase {(5.3.1.5; 5.3.1.9; 5.3.1.18)} · CPC title

• C12P13/08 Primary

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

• C12Y207/01017

  Xylulokinase (2.7.1.17) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title