Microorganisms genetically engineered to have modified N-glycosylation activity
US-9222083-B2 · Dec 29, 2015 · US
Construction of new variants of dextransucrase DSR-S by genetic engineering
US9399765B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9399765-B2 |
| Application number | US-201514798518-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 14, 2015 |
| Priority date | Feb 8, 2006 |
| Publication date | Jul 26, 2016 |
| Grant date | Jul 26, 2016 |
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Abstract
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The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of α-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.
First claim
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Having described the invention, the following is claimed: 1. A method for producing dextrans or isomalto-oligosaccharides with a controlled molar mass, comprising reacting with a polypeptide comprising the amino acid SEQ ID NO: 9 having dextransucrase activity in the presence of sucrose and optionally with at least one acceptor. 2. The method according to claim 1 , in which the reaction takes place in the presence of at least one acceptor selected from glucose,…
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Chemistry & Metallurgy · mapped topic
- C12N9/1051Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Cross-Sectional Technologies · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9399765B2 cover?
- The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the α-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences en…
- Who is the assignee on this patent?
- Centre Nat Rech Scient, Inst Nat De Rech Agronomique, Inst Nat Des Sciences Appliquees De Toulouse
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1051. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 26 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).