Immobilized biologically active entities containing heparin having high biological activity following mechanical manipulation

Patent metadata

Field	Value
Publication number	US-9399085-B2
Application number	US-40974909-A
Country	US
Kind code	B2
Filing date	Mar 24, 2009
Priority date	May 12, 2006
Publication date	Jul 26, 2016
Grant date	Jul 26, 2016

Abstract

The present invention is directed to immobilized biologically active entities that retain significant biological activity following mechanical manipulation of a substrate material to which the entities are immobilized.

First claim

The invention claimed is:

1. A medical device comprising:
a medical device that is capable of being compacted and expanded;
a polymeric substrate material, wherein said polymeric substrate material is fluoropolymeric in composition;
a polymeric covering material attached to at least a portion of a surface of said substrate material;
a first plurality of heparin molecules having anti-thrombin III binding activity end point attached to at least a portion of said polymeric covering material; and
a composition comprising a second plurality of heparin molecules non-covalently combined with said first plurality of heparin molecules;
wherein said first plurality of heparin molecules have an anti-thrombin III binding activity of at least 10 picomoles anti-thrombin III per square centimeter (pmol/cm 2 ) substrate material when measured following compaction of said medical device to 50% of said medical device's original size and expansion of said medical device.

2. The medical device of claim 1 , wherein said fluoropolymeric material is polytetrafluoroethylene.

3. The medical device of claim 1 , further comprising an antiproliferative agent.

4. The medical device of claim 3 , wherein said antiproliferative agent is dexamethasone.

Assignees

• Cleek Robert L
• Daly Michael D
• Pietrzak Krzysztof R
• Gore & Ass

Inventors

• Cleek Robert L
• Daly Michael D
• Pietrzak Krzysztof R

Classifications

• A61L2300/252

  Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines · CPC title

• A61L2300/222

  Steroids, e.g. corticosteroids · CPC title

• A61L2300/236

  Glycosaminoglycans, e.g. heparin, hyaluronic acid, chondroitin · CPC title

• A61L2300/416

  Anti-neoplastic or anti-proliferative or anti-restenosis or anti-angiogenic agents, e.g. paclitaxel, sirolimus · CPC title

• Y10T428/31544

  Addition polymer is perhalogenated · CPC title