Automated system and method of treating tissue samples on slides

Patent metadata

Field	Value
Publication number	US-9395284-B2
Application number	US-201214358480-A
Country	US
Kind code	B2
Filing date	Nov 15, 2012
Priority date	Nov 16, 2011
Publication date	Jul 19, 2016
Grant date	Jul 19, 2016

Abstract

An automated method and system for treating one or more tissue samples disposed on slides, the system comprising: a controller, a plurality of slide treatment modules arranged to receive ones of the slides; at least one fluid dispensing robot configured by the controller to dispense a plurality of reagents to said ones of the slides received in the slide treatment modules via an output nozzle disposed on the at least one fluid dispensing robot to treat said one or more tissue samples respectively; and at least one pumping means for pumping said reagents to the output nozzle of the at least one fluid dispensing robot from a plurality of reagent containers comprising said reagents, wherein the at least one fluid dispensing robot is configured by the controller to dispense said reagents in a predetermined sequence for each of the slide treatment modules to treat the one or more tissue samples disposed on each of the slides independently.

First claim

The invention claimed is:

1. An automated system for treating one or more tissue samples disposed on slides, the system comprising:
a controller;
a plurality of slide treatment modules arranged to receive ones of the slides;
at least one fluid dispensing robot configured by the controller to dispense a plurality of reagents to said ones of the slides received in the slide treatment modules via an output nozzle disposed on the at least one fluid dispensing robot to treat said one or more tissue samples respectively; and
at least one pumping means for pumping said reagents to the output nozzle of the at least one fluid dispensing robot from a plurality of reagent containers comprising said reagents, wherein:
the at least one fluid dispensing robot is configured by the controller to dispense said reagents in a predetermined sequence for each of the slide treatment modules to treat the one or more tissue samples disposed on each of the slides independently,
the at least one fluid dispensing robot comprises a fluid transfer probe (FTP) robot configured by the controller to dispense a plurality of high value reagents to said ones of the slides received in the slide treatment modules via an FTP nozzle disposed on the FTP robot to treat said one or more tissue samples respectively, and
the FTP robot is further configured by the controller to move said slides from an input buffer module to the slide treatment modules using a transport device disposed on the FTP robot to releasably hold the slides.

2. A system as claimed in claim 1 , wherein the at least one fluid dispensing robot comprises at least one bulk fluid robot (BFR) configured by the controller to dispense the plurality of reagents to said ones of the slides received in the slide treatment modules via an output nozzle disposed on the at least one BFR to treat said one or more tissue samples respectively.

3. A system as claimed in claim 2 , wherein the at least one pumping means comprises a plurality of pumping means, each of said pumping means associated with each of the plurality of reagents respectively for pumping said reagents to the output nozzle of the at least one BFR from the plurality of reagent containers comprising said reagents; and
a plurality of reagent lines associated with each of the plurality of reagents which extend from each of the reagent containers via the respective pumping means to the at least one BFR.

4. A system as claimed in claim 3 , wherein the at least one BFR comprises two or more BFRs and the plurality of pumping means comprises pumping means associated with each of the plurality of reagents and with each of the BFRs.

5. A system as claimed in claim 2 , wherein the at least one fluid dispensing robot comprises a fluid transfer probe (FTP) robot configured by the controller to dispense a plurality of high value reagents to said ones of the slides received in the slide treatment modules via an FTP nozzle disposed on the FTP robot to treat said one or more tissue samples respectively.

6. A system as claimed in claim 5 , wherein the at least one BFR and/or the FTP robot are configured by the controller to dispense said reagents and/or said high value reagents in said predetermined sequence to stain said one or more tissue samples disposed on each of the slides independently according to a predetermined staining protocol.

7. A system as claimed in claim 6 , wherein the FTP robot comprises a first syringe pumping means and a second syringe pumping means arranged to aspirate and dispense said high value reagents to/from the FTP nozzle from a plurality of high value reagent containers comprising said high value reagents according to said predetermined sequence.

8. A system as claimed in claim 7 , wherein the first syringe pumping means and the second syringe pumping means are disposed in series and comprise bypass valves operable such that the first and the second syringe pumping means are arranged to aspirate and dispense said high value reagents independently.

9. A system as claimed in claim 7 , wherein the first syringe pumping means and the second syringe pumping means are disposed in parallel such that the first and the second syringe pumping means are arranged to aspirate and dispense said high value reagents independently.

10. A system as claimed in claim 5 , wherein the FTP robot is further configured by the controller to dispense the plurality of reagents from the plurality of reagent containers via the FTP nozzle.

11. A system as claimed in claim 5 , wherein the FTP robot is further configured by the controller to perform one or both of:
a. move said slides from an input buffer module to the slide treatment modules using a transport device disposed on the FTP robot to releasably hold the slides; and
b. move said slides from the slide treatment modules to an output buffer module.

12. A system as claimed in claim 11 , wherein the slides are disposed in one or more slide racks in the input and the output buffer modules and the FTP robot is further configured by the controller to move the slides between the slide racks in the input and the output buffer modules and the slide treatment modules.

13. A system as claimed in claim 12 , wherein the FTP robot is further configured by the controller to move said slides in the slide racks between the input and the output modules and further modules for treating the one or more tissue samples disposed on the slides.

14. A system as claimed in claim 13 , wherein the FTP robot is further configured by the controller to move said slide racks between the input and the output modules and the further modules using the transport device to releasably hold the slide racks.

15. A system as claimed in claim 2 , further comprising sensing means for performing one or both of:
a. sensing amount of said reagents dispensed by the at least one BFR; and
b. sensing amount of said high value reagents dispensed by the FTP robot.

16. An automated method of treating one or more tissue samples disposed on slides, the method comprising:
receiving ones of the slides in a plurality of slide treatment modules;
pumping a plurality of reagents to an output nozzle of at least one fluid dispensing robot from a plurality of reagent containers comprising said reagents using at least one pumping means; and
dispensing the plurality of reagents to said ones of the slides received in the slide treatment modules via the output nozzle disposed on the at least one fluid dispensing robot in a predetermined sequence for each of the slide treatment modules thereby treating the one or more tissue samples disposed on each of the slides independently,
wherein the at least one fluid dispensing robot comprises a fluid transfer probe (FTP) robot configured by the controller to dispense a plurality of high value reagents to said ones of the slides received in the slide treatment modules via an FTP nozzle disposed on the FTP robot to treat said one or more tissue samples respectively, and
wherein the FTP robot is further configured by the controller to move said slides from an input buffer module to the slide treatment modules using a transport device disposed on the FTP robot to releasably hold the slides.

17. An automated tissue sample treatment apparatus for treating one or more tissue samples disposed on slides, the apparatus comprising:
a plurality of slide treatment modules arranged to receive ones of the slides;
at least one fluid dispensing robot configured by the controller to dispense a plurality of reagents to said ones of the slides received in the slide treatment modules via an outpu

Assignees

• Leica Biosystems Melbourne Pty

Inventors

• Favaloro Anthony
• Restorick Luke
• Drummond Michael Houston
• Bagnato Stephen John

Classifications

• G01N35/0099

  comprising robots or similar manipulators (robots per se B25J) · CPC title

• G01N1/312 Primary

  for samples mounted on planar substrates · CPC title

• G01N35/1002

  Reagent dispensers · CPC title