Cyclic peptide production

Patent metadata

Field	Value
Publication number	US-9394561-B2
Application number	US-201214363561-A
Country	US
Kind code	B2
Filing date	Dec 7, 2012
Priority date	Dec 7, 2011
Publication date	Jul 19, 2016
Grant date	Jul 19, 2016

Abstract

An enzyme useful for producing cyclic peptides from linear peptide precursors and a gene encoding the enzyme are described. The enzyme is particularly useful for producing segetalins from linear presegetalin precursors. The linear presegetalin precursors may be derived from other linear presegetalin precursors farther upstream in the biosynthetic synthesis of the segetalin.

First claim

The invention claimed is:

1. An recombinant polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 2 and has a function of peptide cyclization, wherein the recombinant polypeptide is recombinantly expressed in a microorganism.

2. The recombinant polypeptide according to claim 1 , wherein the amino acid sequence has at least 99% sequence identity to the amino acid sequence as set forth in SEQ ID NO: 2.

3. The recombinant polypeptide according to claim 1 , wherein the amino acid sequence is as set forth in SEQ ID NO: 2.

4. A process of producing a cyclic peptide, the process comprising contacting a suitable linear peptide precursor of the cyclic peptide with the polypeptide of claim 1 to produce the cyclic peptide from the linear peptide precursor.

5. The process according to claim 1 , wherein the amino acid sequence is as set forth in SEQ ID NO: 2.

6. The process according to claim 4 , wherein the linear peptide precursor is provided to a microbial host cell transformed or transfected with a nucleic acid molecule encoding the recombinant polypeptide according to claim 1 .

7. The process according to claim 4 , wherein the cyclic peptide is segetalin A.

8. The process according to claim 4 , wherein the cyclic peptide comprises the amino acid sequence as set forth in SEQ ID NO: 77.

9. The process according to claim 4 , wherein the linear peptide precursor is produced by a recombinant organism.

Assignees

• Nat Res Council Canada

Inventors

• Barber Carla Jann Siobhan
• Reed Darwin Wilfred
• Condie Janet Anne
• Chiwocha Sheila Diniwe Sherin
• Covello Patrick Smithers

Classifications

• C12P21/06 Primary

  produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title

• C12N15/63

  Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title

• C07K7/64

  Cyclic peptides containing only normal peptide links · CPC title

• C12N9/48

  acting on peptide bonds (3.4) · CPC title

• C12N15/8257

  for the production of primary gene products, e.g. pharmaceutical products, interferon · CPC title