PHI-4 polypeptides and methods for their use

Patent metadata

Field	Value
Publication number	US-9394345-B2
Application number	US-201313839702-A
Country	US
Kind code	B2
Filing date	Mar 15, 2013
Priority date	Mar 15, 2013
Publication date	Jul 19, 2016
Grant date	Jul 19, 2016

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed:

1. A variant polypeptide comprising the amino acid sequence of SEQ ID NO: 3
wherein
Xaa at position 2 is Ala or Arg;
Xaa at position 9 is Gln, Lys or Glu;
Xaa at position 14 is Pro or Ala;
Xaa at position 16 is Val or Asp;
Xaa at position 19 is Met or Leu;
Xaa at position 22 is Gly or Ser;
Xaa at position 24 is Asp, Asn or Gln;
Xaa at position 36 is Leu or Met;
Xaa at position 42 is Asp, Asn or Gln;
Xaa at position 43 is Phe or Glu;
Xaa at position 46 is Glu, Asp, Asn or Gly;
Xaa at position 50 is Ile or Val;
Xaa at position 51 is Glu or Gin;
Xaa at position 55 is Arg or Lys;
Xaa at position 56 is Ser or Thr;
Xaa at position 57 is Tyr or Phe;
Xaa at position 58 is Thr or Ser;
Xaa at position 61 is Arg, Lys or Glu;
Xaa at position 73 is Phe or Tyr;
Xaa at position 74 is Lys, Glu, Gly, Arg, Met, Leu, His or Asp;
Xaa at position 76 is Asp or Gln;
Xaa at position 79 is Lys or Glu;
Xaa at position 80 is Glu or Ser;
Xaa at position 82 is Glu, Ile, Leu, Tyr or Gln;
Xaa at position 83 is Glu or Gln;
Xaa at position 84 is Tyr or Phe;
Xaa at position 86 is Glu or Gln;
Xaa at position 87 is Lys or Gln;
Xaa at position 88 is Met, Ile or Leu;
Xaa at position 90 is Gln or Glu;
Xaa at position 94 is Val or Ile;
Xaa at position 97 is Arg, Asn, Asp, Glu, Gln, Gly or Ser;
Xaa at position 98 is Tyr or Phe;
Xaa at position 99 is Lys, Leu, Tyr, Ile, Met, Phe, Cys, Val or Asn;
Xaa at position 103 is Ala or Gly;
Xaa at position 105 is Leu or Ile;
Xaa at position 109 is Phe, Lys, Gly, Met, Ser, Asp, Asn, Glu, Cys, Ala or Arg;
Xaa at position 112 is Thr or Ser;
Xaa at position 113 is Asp, Glu or Met;
Xaa at position 117 is Thr or Ser;
Xaa at position 121 is Tyr or Phe;
Xaa at position 127 is Ala or Thr;
Xaa at position 142 is Arg or Glu;
Xaa at position 146 is Arg or Gln;
Xaa at position 147 is Arg, Glu or Gln;
Xaa at position 148 is Asp, Phe, Pro, Val, Glu, His, Trp, Ala, Arg, Leu, Ser, Gln or Gly;
Xaa at position 149 is Phe or Val;
Xaa at position 150 is Arg, Gln or Glu;
Xaa at position 151 is Asp, Ser, Ala, Asn, Trp, Val, Gln, Cys, Met, Leu, Arg or Glu;
Xaa at position 153 is Leu or Ile;
Xaa at position 154 is Asn or Asp;
Xaa at position 155 is Asn or Lys;
Xaa at position 159 is Pro or Asp;
Xaa at position 162 is Glu, Asp or Gln;
Xaa at position 165 is Lys, Glu, Gln, Pro, Thr, Ala, Leu, Gly, Asp, Val, His, Ile, Met, Trp, Phe, Tyr or Arg;
Xaa at position 166 is Arg or Gln;
Xaa at position 167 is Tyr, Trp or Cys;
Xaa at position 170 is Tyr or His;
Xaa at position 171 is Tyr or Phe;
Xaa at position 172 is Ile, Leu or Val;
Xaa at position 173 is Ser or Ala;
Xaa at position 174 is Glu or Gln;
Xaa at position 182 is Asp or Gln;
Xaa at position 183 is Tyr or Val;
Xaa at position 184 is Ser or Thr;
Xaa at position 185 is Ala or Ser;
Xaa at position 189 is Thr, Lys or Ile;
Xaa at position 191 is Lys or Gln;
Xaa at position 193 is Asp or Asn;
Xaa at position 196 is Gln, Lys, Asn, Asp, Glu, Ala, Ile or Arg;
Xaa at position 202 is Ala or Val;
Xaa at position 203 is Glu, Thr or His;
Xaa at position 204 is Met or Ala;
Xaa at position 206 is Tyr or Phe;
Xaa at position 207 is Lys or Gln;
Xaa at position 209 is Leu or Pro;
Xaa at position 210 is Val or Ile;
Xaa at position 214 is Lys, Ser or Gln;
Xaa at position 216 is Glu, Gln, Phe, Val, Tyr or Arg;
Xaa at position 220 is Glu, His, Asp, Thr, Tyr, Val, Ser, Gln, Arg, Trp, Met, Ala, Phe, Ile, Leu, Cys or Asn;
Xaa at position 229 is Arg or Glu;
Xaa at position 230 is Ser or Glu;
Xaa at position 231 is Asn or Ser;
Xaa at position 236 is Leu or Pro;
Xaa at position 245 is Met or Leu;
Xaa at position 247 is Asp or Tyr;
Xaa at position 256 is Gln, Lys or Glu;
Xaa at position 257 is Gln, Ile, Glu, Cys, Ser, His, Trp or Met;
Xaa at position 261 is Gln, Glu or Lys;
Xaa at position 264 is Glu or Gln;
Xaa at position 268 is Asp or Asn;
Xaa at position 276 is Ser or Ala;
Xaa at position 278 is Glu, Asn or Gln;
Xaa at position 281 is Gln, Lys or Glu;
Xaa at position 282 is Pro or Gly;
Xaa at position 284 is Trp or Arg;
Xaa at position 287 is Ala or Cys;
Xaa at position 289 is Lys, Leu, Val, Pro, Glu, Gln, Tyr, Thr, Asp, Phe, Ser, Met, Arg, Trp, Ile, His, Asn, Cys, Gly or Ala;
Xaa at position 291 is Glu or Gln;
Xaa at position 292 is Arg or Gln;
Xaa at position 293 is Arg, Glu or Gln;
Xaa at position 294 is Val or Ala;
Xaa at position 296 is Leu or Ile;
Xaa at position 297 is Glu or Gln;
Xaa at position 298 is Asp or Gln;
Xaa at position 300 is Phe or Tyr;
Xaa at position 302 is Glu or Gln;
Xaa at position 303 is Phe or Tyr;
Xaa at position 305 is Lys or Gln;
Xaa at position 306 is Gln or Lys;
Xaa at position 309 is Gln, Lys or Glu;
Xaa at position 313 is Lys, Gln or Arg;
Xaa at position 316 is Lys or Gln;
Xaa at position 328 is Lys, Glu or Gln;
Xaa at position 331 is Glu, Asn or Gln;
Xaa at position 333 is Ser, Arg, Gly, Lys, Val, Asn, Ala, His, Gln, Thr, Asp, Ile, Leu, Cys or Glu;
Xaa at position 334 is Gly, Arg, Lys, Ile or Trp;
Xaa at position 335 is Ser or Ala;
Xaa at position 336 is Gly or Ala;
Xaa at position 337 is Ala, Val or Gly;
Xaa at position 338 is Ser, His, Val, Lys, Ala, Gly, Thr, Ile, Glu, Met, Arg, Pro, Asp, Asn or Leu;
Xaa at position 339 is Glu, Asn, Gln, Ile, Pro, Met, Ser, Ala, Cys, Phe, Val, Leu, Asp, Trp, His or Arg;
Xaa at position 341 is Leu or Val;
Xaa at position 342 is Ala, Ser or Val;
Xaa at position 343 is Val or Ile;
Xaa at position 344 is Phe or Trp;
Xaa at position 345 is Asn or His;
Xaa at position 346 is Pro or Ala;
Xaa at position 350 is Asn or Ser;
Xaa at position 351 is Gly or Val;
Xaa at position 354 is Met or Leu;
Xaa at position 355 is Val, Ile or Leu;
Xaa at position 359 is Gly or Ala;
Xaa at position 362 is Asn or Ser;
Xaa at position 364 is Ala or Ser;
Xaa at position 371 is Ala, Gly or Thr;
Xaa at position 374 is Phe or Ile;
Xaa at position 375 is Lys or Arg;
Xaa at position 380 is Leu or Gly;
Xaa at position 382 is Val, Asp or Leu;
Xaa at position 383 is Leu, Ile or Val;
Xaa at position 384 is Lys, Ala or Gly;
Xaa at position 385 is Ala or Gly;
Xaa at position 389 is Trp or Tyr;
Xaa at position 391 is Arg, Leu, Glu, Gln or Asp;
Xaa at position 395 is Asp or Cys;
Xaa at position 396 is Ala, Leu, Lys, Asn, Gly, Ile, Met, Arg, Tyr, Gln or His;
Xaa at position 397 is Gly, Arg or Ala;
Xaa at position 398 is Ser, Gln or Cys;
Xaa at position 401 is Ser, His, Pro, Gly, Lys, Val, Arg, Ile, Asn, Phe, Thr, Ala, Asp, Met, Gln or Glu;
Xaa at position 402 is Lys, Phe, His, Arg, Trp, Gly, Asn, Leu, Tyr, Thr, Val, Met, Pro or Ala;
Xaa at position 403 is Asp, Tyr, Trp, Phe or Glu;
Xaa at position 405 is Ala or Ser;
Xaa at position 409 is Ala or Pro;
Xaa at position 410 is Ile or Val;
Xaa at position 411 is Pro or Ala;
Xaa at position 412 is Pro or Ala;
Xaa at position 416 is Arg, Glu or Gln;
Xaa at position 417 is Ala, Ser or Cys;
Xaa at position 418 is Leu or Met;
Xaa at position 422 is Met or Val;
Xaa at position 426 is Thr or Ser;
Xaa at position 436 is Asp or Lys;
Xaa at position 437 is Tyr or Val;
Xaa at position 438 is Val or Arg;
Xaa at position 440 is Val or Leu;
Xaa at position 442 is Gln, Lys or Glu;
Xaa at position 445 is Cys, Leu or Thr;
Xaa at position 447 is Asp, Lys, Tyr, Ser, Glu, Ile, Gly, Pro, Leu, Phe, Trp or Thr;
Xaa at position 448 is Val or Ala;
Xaa at position 449 is Gln or Glu;
Xaa at position 452 is Gln, Lys or Glu;
Xaa at position 453 is Asn or Asp;
Xaa at position 454 is Arg, Tyr, Met, Ser, Val, Ile, Lys, Phe, Trp, Gln, Gly, His, Asp, Leu, Thr, Pro or Asn;
Xaa at position 455 is Val or Ile;
Xaa at position 457 is Trp or Asn;
Xaa at position 459 is Lys, Met, Val, Trp, Gln, Ile, Thr, Ser, His, Cys, Tyr, Pro, As

Assignees

• Pioneer Hi Bred Int

Inventors

• Cong Ruth
• Hou Jingtong
• Hou Zhenglin
• Patten Phillip
• Yamamoto Takashi

Classifications

• A01N43/50

  1,3-Diazoles; Hydrogenated 1,3-diazoles · CPC title

• C07K14/195 Primary

  from bacteria · CPC title

• C07K14/21

  from Pseudomonadaceae (F) · CPC title

• C07K14/24

  from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia · CPC title

• A01N37/46

  N-acyl derivatives · CPC title