Hemostat reconstitution methods and devices
US-12343385-B2 · Jul 1, 2025 · US
US9393266B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9393266-B2 |
| Application number | US-17511908-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 17, 2008 |
| Priority date | Jul 17, 2007 |
| Publication date | Jul 19, 2016 |
| Grant date | Jul 19, 2016 |
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Bags and/or dark circles around the eyes of individuals afflicted therewith are reduced by administering to such individuals thus effective amounts of at least one extract of a non-photosynthetic and non-fruiting filamentous bacterium cultured in a medium which includes at least one non-sulfurous mineral and/or thermal water, e.g., an extract derived from the bacterium Vitreoscilla filiformis , in particular the strain ATCC 15551, cultured in a medium enriched with water from La Roche Posay.
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What is claimed is: 1. A cosmetic/dermatological method for reducing the bags and/or dark circles around the eyes of an individual afflicted therewith, the method comprising: making a culture medium, which comprises adding (a) osmosed and/or distilled waters and (b) a second water comprising a total concentration of carbonates or bicarbonates of at least 150 mg/l, at least 6 mg/l of silicon oxide, at least 100 mg/l calcium ions, and at least 4 mg/l sulfates, wherein (b) is added in the range of 0.5 to 50 percent based on the amount of osmosed and/or distilled water of (a); culturing a non-photosynthetic and non-fruiting filamentous bacterium in said medium to produce a culture of non-photosynthetic bacterium in said medium; preparing a composition comprising between 0.001% and 20% by weight of an extract of said cultured bacterium; and topically administering to said individual an effective amount of said composition; wherein said non-photosynthetic and non-fruiting filamentous bacterium is selected from the group consisting of Vitreoscilla filiformis, Vitreocilla beggiatoides, Beggiatoa alba, Flexithrix dorotheae, Leucothrix mucor , and Speaerotilus natans. 2. The cosmetic/dermatological method as defined by claim 1 , wherein the method reduces hyperpigmentation of the suborbital region and/or lightening dark circles afflicting said individual. 3. The cosmetic/dermatological method as defined by claim 1 , wherein the method renders the complexion of the region of the dark circles afflicting said individual uniform with the remainder of his or her face. 4. The cosmetic/dermatological method as defined by claim 1 , wherein said extract is comprised in a concealer eye contour cream, a concealer eye contour gel or a concealer stick. 5. The cosmetic/dermatological method as defined by claim 1 , said medium further comprising autolyzed yeast extract, plant peptone, anhydrous glucose, Heller microelements and calcium chloride. 6. The cosmetic/dermatological method as defined by claim 1 , said at least one extract comprising Se, Sr and Zn. 7. A cosmetic/dermatological method for treating the vascular component of a disorder of the eye contour of an individual afflicted therewith, the method comprising: making a culture medium, which comprises adding (a) osmosed and/or distilled waters and (b) a second water comprising a total concentration of carbonates or bicarbonates of at least 150 mg/l, at least 6 mg/l of silicon oxide, at least 100 mg/l calcium ions, and at least 4 mg/l sulfates, wherein (b) is added in the range of 0.5 to 50 percent based on the amount of osmosed and/or distilled water of (a); culturing a non-photosynthetic and non-fruiting filamentous bacterium in said medium to produce a culture of non-photosynthetic bacterium in said medium; preparing a composition comprising between 0.001% and 20% by weight of an extract of said cultured bacterium; and topically administering to said individual an effective amount of said composition; wherein said non-photosynthetic and non-fruiting filamentous bacterium is selected from the group consisting of Vitreoscilla filiformis, Vitreocilla beggiatoides, Beggiatoa alba, Flexithrix dorotheae, Leucothrix mucor , and Speaerotilus natans.
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