Solid substrate comprising array of dendrons and methods for using the same

Patent metadata

Field	Value
Publication number	US-9389227-B2
Application number	US-10279408-A
Country	US
Kind code	B2
Filing date	Apr 14, 2008
Priority date	Sep 5, 2001
Publication date	Jul 12, 2016
Grant date	Jul 12, 2016

Abstract

The present invention provides solid supports comprising a surface bound array of dendrons and methods for using the same.

First claim

What is claimed is:

1. An array of dendrons, said array comprising a solid support having at least a first surface; and a plurality of conically shaped dendrons covalently attached to said first surface of said solid support, wherein each of said dendron is of the formula:
Z—R 1 -Q 1 -{(R 3 -Q 3 ) b -[(R 4 -Q 4 ) c -(R 5 —Y) x ] y } 3

wherein
c is 0 or 1;
x is 1 when c is 0 or when c is 1, x is 3;
y is 1 when b is 0 or when b is 1, y is 3;
Z is X-O-C(═O)—NH—, wherein X is a nucleic acid probe;
R 1 is —(CH 2 ) 3 —C(═O)—NH—;
Q 1 , Q 3 and Q 4 are C;
R 3 is —CH 2 —O—(CH 2 ) 2 —C(═O)—NH—(CH 2 ) 3 —C(═O)—NH—;
R 4 is —CH 2 —O—(CH 2 ) 2 —C(═O)—NH—;
R 5 is —CH 2 —O—(CH 2 ) 2 —;
Y is a terminal functional group moiety of the formula —COOH;

wherein a plurality of said terminal functional groups on each of said dendron are covalently attached to said first surface of said solid support, and wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm, and wherein a discrimination efficiency of the probe is at least 80%.

2. The array according to claim 1 , wherein b is 0.

3. The array according to claim 1 , wherein b is 1.

4. The array according to claim 3 , wherein c is 1.

5. An array of dendrons, said array comprising a solid support having at least a first surface; and a plurality of conically shaped dendrons covalently attached to said first surface of said solid support, wherein each of said dendron is of the formula:
Z—R 1 -Q 1 -[(R 4 -Q 4 ) c -(R 5 —Y) x ] 3   I

wherein
c is 0 or 1;
x is 1 when c is 0 or when c is 1, x is 3;
Z is X-O-C(═O)—NH—, wherein X is a probe;
R 1 is —(CH 2 ) 3 —C(═O)—NH—;
Q 1 and Q 4 are C;
R 4 is —CH 2 —O—(CH 2 ) 2 —C(═O)—NH—;
R 5 is —CH 2 —O—(CH 2 ) 2 —;
Y is a terminal functional group moiety of the formula —COOH;

wherein a plurality of said terminal functional groups on each of said dendron are covalently attached to said first surface of said solid support, and wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm, and wherein the discrimination efficiency of the probe is at least 80%.

6. The array according to claim 5 , wherein c is 0.

7. The array according to claim 5 , wherein c is 1.

Assignees

• Park Joon Won
• Hong Bong Jin
• Posco
• Postech Foundation

Inventors

• Park Joon Won
• Hong Bong Jin

Classifications

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• B01J2219/00637

  by coating it with another layer · CPC title

• B01J2219/00605

  the compounds being directly bound or immobilised to solid supports · CPC title

• C40B50/18

  using a particular method of attachment to the solid support · CPC title

• B01J2219/00608

  DNA chips · CPC title