Biocatalysts and methods for synthesizing derivatives of tryptamine and tryptamine analogs

Patent metadata

Field	Value
Publication number	US-9388395-B2
Application number	US-201514828839-A
Country	US
Kind code	B2
Filing date	Aug 18, 2015
Priority date	Mar 23, 2012
Publication date	Jul 12, 2016
Grant date	Jul 12, 2016

Abstract

The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

First claim

What is claimed is:

1. An engineered polypeptide having transaminase activity, comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 4 and substitution at least at positions X14 and X113 as compared to SEQ ID NO:4.

2. The engineered polypeptide of claim 1 , further comprising residue differences at the residue positions X26; X33; X41; X47; X70; X88; X107; X132; X148; X173; X203; X250; X284; X315; X346; X395; X400; X419; X423; X448; and X451 are selected from X26R; X33T; X41L; X47N; X70A; X88A; X88L; X107P; X132F; X148Q; X148R; X173A; X2035; X250A; X284A; X315G; X346L; X395P; X400G; X419S; X423I; X448E; and X451D.

3. The engineered polypeptide of claim 2 , in which the amino acid sequence further comprises one or more residue differences selected from: X57F; X168K; X420N; and X424V.

4. The engineered polypeptide of claim 1 , in which the amino acid sequence comprises at least one or more additional residue differences selected from: X26R; X315/D; X86D; X163I/L/R/V; X284A; X315G; X398L/V/W; and X400G.

5. The engineered polypeptide of claim 1 , in which the amino acid sequence comprises the substitution X14V, X113L/V and residue differences selected from:
X163I/L/R/V;
X86D and X4000;
X57F/Y and X163I/UR/V;
X57F/Y and X398L/V/W;
X163I/L/R/V, X284A, and X424V;
X31S, X57F/Y, X163I/L/R/V, X3150, X346L, and X398L/V/W;
X163L, X284A, and X424V;
X26R, X163L, X284A, and X4000;
X26R and X88L;
X57F, X163L, X168K, X314N, X3150, X346L, and X398V;
X163L, X173A, X4000, and X420N;
X163L and X284A;
X26R, X163L, X284A, and X4000; and
X33T, X57F, and X163L.

6. The engineered polypeptide of claim 1 , in which the transaminase has at least 1.2 fold increased stability as compared to the polypeptide of SEQ ID NO:4.

7. The engineered polypeptide of claim 1 , in which the amino acid sequence comprises a sequence selected from: SEQ ID NO:16.

8. A process for preparing a compound of formula (I),

wherein
R′ is selected from the group consisting of hydrogen, carboxy, carboxy(C 1 -C 6 )alkyl, optionally substituted (C 1 -C 6 )alkyl, optionally substituted (C 2 -C 6 )alkenyl, optionally substituted alkyloxycarbonyl, optionally substituted arylcarbonyl, optionally substituted arylsulfonyl, and a protecting group;
R 2 is selected from the group consisting of hydrogen, oxo, halo, hydroxy, amino, thio, optionally substituted (C 1 -C 6 )alkyl, (C 1 -C 6 )alkylamino, (C 1 -C 6 )dialkylamino, (C 1 -C 6 )alkylthio, (C 1 -C 6 )alkylsulfonyl, (C 1 -C 6 )alkylsulfinyl, and optionally substituted (C 1 -C 6 )alkyloxy;
R 4 , R 6 and R 7 are each, independently of the others, selected from the group consisting of hydrogen, halo, hydroxy, amino, carboxy, cyano, nitro, thio, optionally substituted (C 1 -C 6 )alkyl, hydroxy(C 1 -C 6 )alkyl, optionally substituted (C 1 -C 6 )alkyloxy, optionally substituted (C 1 -C 6 )alkylamino, optionally substituted (C 1 -C 6 )dialkylamino, optionally substituted (C 1 -C 6 )alkylthio, optionally substituted (C 1 -C 6 )alkylsulfonyl, optionally substituted (C 1 -C 6 )alkylsulfinyl, carboxy(C 1 -C 6 )alkyl, (C 1 -C 6 )alkyloxycarbonyl, (C 1 -C 6 )alkylcarbonyloxy, optionally substituted aminocarbonyl, aminocarbonyl(C 1 -C 6 )alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted aryloxy, optionally substituted arylamino, optionally substituted arylthio, optionally substituted arylsulfonyl, optionally substituted arylsulfinyl, optionally substituted aryloxycarbonyl, optionally substituted arylcarbonyloxy, optionally substituted heteroaryloxy, optionally substituted heteroarylamino, optionally substituted heteroarylthio, optionally substituted heteroarylsulfonyl, optionally substituted heteroarylsulfinyl, optionally substituted heteroaryloxycarbonyl, optionally substituted heteroarylcarbonyloxy, alkylaminosulfonyl(C 1 -C 6 )alkyl, arylsulfonyl(C 1 -C 6 )alkyl, and heteroarylsulfonyl(C 1 -C 6 )alkyl;
R 5 is selected from the group consisting of hydrogen, halo, hydroxy, amino, carboxy, cyano, nitro, thio, optionally substituted (C 1 -C 6 )alkyl, hydroxy(C 1 -C 6 )alkyl, optionally substituted (C 1 -C 6 )alkyloxy, optionally substituted (C 1 -C 6 )alkylamino, optionally substituted (C 1 -C 6 )dialkylamino, optionally substituted (C 1 -C 6 )alkylthio, optionally substituted (C 1 -C 6 )alkylsulfonyl, optionally substituted (C 1 -C 6 )alkylsulfinyl, carboxy(C 1 -C 6 )alkyl, (C 1 -C 6 )alkyloxycarbonyl, (C 1 -C 6 )alkylcarbonyloxy, optionally substituted aminocarbonyl, aminocarbonyl(C 1 -C 6 )alkyl, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, optionally substituted heteroaryl, optionally substituted aryloxy, optionally substituted arylamino, optionally substituted arylthio, optionally substituted arylsulfonyl, optionally substituted arylsulfinyl, optionally substituted aryloxycarbonyl, optionally substituted arylcarbonyloxy, optionally substituted heteroaryloxy, optionally substituted heteroarylamino, optionally substituted heteroarylthio, optionally substituted heteroarylsulfonyl, optionally substituted heteroarylsulfinyl, optionally substituted heteroaryloxycarbonyl, optionally substituted heteroarylcarbonyloxy, alkylaminosulfonyl(C 1 -C 6 )alkyl, arylsulfonyl(C 1 -C 6 )alkyl, and heteroarylsulfonyl(C 1 -C 6 )alkyl, or together with R 4 forms a 5 to 8 membered optionally substituted cycloalkyl or optionally substituted heterocyclic ring;
R 8 is selected from the group consisting of optionally substituted (C 1 -C 6 ) alkyl, optionally substituted (C 1 -C 6 )alkyloxy, optionally substituted cycloalkyl, optionally substituted heterocycloalkyl, optionally substituted aryl, and optionally substituted heteroaryl, or together with R 2 forms an optionally substituted 5 to 8 membered cycloalkyl or heterocyclic ring; and
n is 1 or 2;
with the provisos that
(a) when R 2 is hydrogen, then at least one of the following applies:
(i) R 1 is not hydrogen, methyl, 4-(methyloxy)phenylcarbonyl-, 4-(trifluoromethyloxy)phenylsulfonyl-, 3-bromophenylcarbonyl-, 3-aminopropyl-, or 3-(methylcarbonylamino)propyl-;
(ii) R 4 and R 7 are each, independently of the other, not hydrogen or chloro;
(iii) R 5 is not hydrogen, hydroxy, methyl, methyloxy, fluoro, chloro, trifluoromethyl, or cyano;
(iv) R 6 is not hydrogen, hydroxy, methyloxy, fluoro or chloro; or
(v) R 8 is not methyl, ethyl, hydroxymethyl, or trifluoromethyl-; and

(b) when n is 1, R 2 and R 8 together form a cyclohexyl ring, and R 1 , R 4 , R 6 , and R 7 are hydrogen, then R 5 is not fluoro;
comprising contacting the substrate compound of formula (II),

wherein R 1 , R 2 , R 4 , R 5 , R 6 , R 7 , R 8 and n are as defined above,
in presence of an amino donor under suitable reaction conditions with an engineered transaminase polypeptide of claim 1 .

9. The process of claim 8 , in which the compound of formula (I) comprises the compound of formula (IS),

having the indicated stereochemistry at the carbon atom marked with an *, wherein compound (IS) is formed in enantiomeric excess.

10. A process for preparing a compound of formula (Ia):

wherein,
R 1 is selected from the group consisting of hydrogen, carboxy, carboxy(C

Assignees

• Codexis Inc

Inventors

• Nazor Jovana
• Smith Derek
• Crowe Michael
• Song Shiwei
• Collier Steven J

Classifications

• C12N9/1096 Primary

  transferring nitrogenous groups (2.6) · CPC title

• C12P17/10

  Nitrogen as only ring hetero atom · CPC title

• C12Y206/01018

  Beta-alanine-pyruvate transaminase (2.6.1.18) · CPC title

• C12Y206/01

  Transaminases (2.6.1) · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title