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Methods for making cells with an extra-embryonic endodermal precursor phenotype
US9388388B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9388388-B2 |
| Application number | US-201213996248-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 30, 2012 |
| Priority date | Jan 31, 2011 |
| Publication date | Jul 12, 2016 |
| Grant date | Jul 12, 2016 |
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Abstract
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The invention is directed to methods for making cells in vitro that have an extra-embryonic endodermal precursor phenotype. The method involves culturing cells from blastocysts under specific defined conditions.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method to produce extra-embryonic endodermal precursor cells that express extra-embryonic endodermal precursor genes, the method comprising culturing a blastocyst in a culture medium containing platelet-derived growth factor under conditions of reduced oxygen to produce the extra-embryonic endodermal precursor cells wherein the conditions for reduced oxygen include conditions less than about 20%. 2. The method of claim 1 wherein the oxygen concentration is about 10% or less. 3. The method of claim 2 wherein the oxygen concentration is about 5%. 4. The method of claim 1 , wherein the extra-embryonic precursor cells express one or more transcription factors selected from the group consisting of oct4, rex-1, Ecats, Gata4, Gata6, Sox7, Sox17, Foxa2, HNF1Beta, and HNF4alpha. 5. The method of any one of claims 1 - 3 and 4 wherein the blastocysts are cultured without a cellular feeder layer. 6. The method of any one of claims 1 - 3 and 4 , wherein the serum concentration is about 2% to about 5%. 7. The method of any one of claims 1 - 3 and 4 , wherein the serum concentration is about 2%.
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Classifications
for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics · CPC title
Atmosphere, e.g. low oxygen conditions · CPC title
Platelet-derived growth factor [PDGF] · CPC title
- C12N5/0605Primary
Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly · CPC title
- C12N5/0696Primary
Artificially induced pluripotent stem cells, e.g. iPS · CPC title
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Frequently asked questions
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- What does patent US9388388B2 cover?
- The invention is directed to methods for making cells in vitro that have an extra-embryonic endodermal precursor phenotype. The method involves culturing cells from blastocysts under specific defined conditions.
- Who is the assignee on this patent?
- Verfaillie Catherine M, Binas Bert, Univ Leuven Kath, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0605. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 12 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).