Electrophoresis gel cassette

Patent metadata

Field	Value
Publication number	US-9383335-B2
Application number	US-201314403613-A
Country	US
Kind code	B2
Filing date	May 31, 2013
Priority date	May 31, 2012
Publication date	Jul 5, 2016
Grant date	Jul 5, 2016

Abstract

Electrophoresis gel cassette comprising a first and a second face wall member and one or more side wall members defining a gel compartment for a gel member with a first and second face, wherein at least a section of each one of the first and second face wall member is removable to expose a section of both the first and second face of the gel member essentially corresponding to the separation zone while the gel member is preserved in the cassette.

First claim

What is claimed is:

1. An electrophoresis gel cassette comprising a first face wall member, a second face wall member, and one or more side wall members defining a gel compartment for a gel member with a first face and a second face, wherein at least a section of each of the first face wall member and the second face wall member is removable to expose a section of both the first face and the second face of the gel member essentially corresponding to a separation zone while the gel member is preserved in the cassette, wherein at least one of the removable sections of the first face wall member and the second face wall member is formed of two or more laminated layers of plastic film, the two or more laminated layers of plastic film comprising a first layer and a second layer, wherein the first layer faces the gel compartment and has one or more openings formed therein which are covered by one or more peelable sections of the second layer.

2. The electrophoresis gel cassette according to claim 1 , wherein the side wall members are formed integrally with one of the face wall members to form a cassette body.

3. The electrophoresis gel cassette according to claim 1 , wherein the removable sections of the first face wall member and the second face wall member are provided with low gel adhesion properties with respect to the gel member.

4. The electrophoresis gel cassette according to claim 1 , wherein at least one of the first face wall member and the second face wall member is provided with one or more removable sections arranged to expose a buffer contact section of the gel member.

5. The electrophoresis gel cassette according to claim 1 , further comprising integrated buffer compartments.

6. The electrophoresis gel cassette according to claim 1 , wherein at least one of the removable sections of the first face wall member and the second face wall member is formed as a rigid lid.

7. The electrophoresis gel cassette according to claim 6 , wherein the first layer comprises a PET film with adhesive EVA layers applied to a first face and a second face of the first layer, and wherein the second layer comprises a PET film.

8. The electrophoresis gel cassette according to claim 1 , wherein the first layer comprises a rigid polymer film with adhesive layers applied to a first face and a second face of the first layer, and wherein the second layer comprises a rigid polymer film.

9. The electrophoresis gel cassette according to claim 1 , further comprising a precast gel.

10. The electrophoresis gel cassette according to claim 1 , wherein at least one of the first face wall member and the second face wall member comprises an optically transparent material to allow imaging of the gel member without opening the cassette.

11. The electrophoresis gel cassette according to claim 1 , further comprising an alignment structure defining a positional reference for alignment of the cassette.

12. The electrophoresis gel cassette according to claim 1 , further comprising an identification code.

13. The electrophoresis gel cassette according to claim 5 , further comprising electrodes.

Assignees

• Ge Healthcare Bio Sciences Ab

Inventors

• Bjorkesten Lennart
• Salven Owe
• Larsson Camilla
• Bjerneld Erik J
• Edlund Sofia
• Ostlin Henrik
• Sjolander Stefan
• Stridsberg-Friden Kajsa
• Ronn Ola
• Oliviusson Peter
• Bjorkman Henrik

Classifications

• G01N27/44704 Primary

  Details; Accessories · CPC title

• G01N27/44756

  Apparatus specially adapted therefor · CPC title

• G01N27/447 Primary

  using electrophoresis · CPC title