Apparatus for separating magnetic particles from liquids containing said particles, and an array of vessels suitable for use with such an apparatus

Patent metadata

Field	Value
Publication number	US-9381519-B2
Application number	US-201313900389-A
Country	US
Kind code	B2
Filing date	May 22, 2013
Priority date	Mar 31, 2006
Publication date	Jul 5, 2016
Grant date	Jul 5, 2016

Abstract

An apparatus for separating magnetic particles from a liquid which contains said particles, said liquid being contained in an elongated vessel ( 11 ) having a length axis ( 12 ), said vessel being arranged in a vessel holder ( 13 ) with its length axis ( 12 ) in a substantially vertical position, said vessel ( 11 ) having a bottom and a tapered cross-section that diminishes towards the bottom of the vessel and a side wall ( 14 ) which has an outer surface which forms an angle with the length axis ( 12 ) of said vessel ( 11 ). This apparatus comprises a magnet ( 15 ) having a plane outer surface ( 16 ) and being adapted for being moved by transport means ( 17 ) along a motion path ( 25 ), said magnet ( 15 ) and said transport means ( 17 ) being so arranged with respect to said vessel ( 11 ) that over a portion of said motion path ( 25 ) said plane outer surface ( 16 ) of said magnet is in contact with a portion of said outer surface of said side wall ( 14 ) of said vessel ( 11 ), and transport means ( 17 ) for moving said magnet ( 15 ) between a first predetermined position and a second predetermined position along said motion path ( 25 ).

First claim

What is claimed is:

1. An apparatus for separating magnetic particles from a liquid which contains said particles, said apparatus comprising:
(a) a vessel array comprising a plurality of elongated vessels, each elongated vessel comprising a length axis, an upper portion, a lower portion comprising a central part and a bottom part, and a side wall extending between the upper and lower portions, wherein the lower portion comprises a cross-section tapered in diameter towards the bottom part of the vessel relative to a diameter of the upper portion, and the side wall includes an outer surface comprising a conical shape, wherein said vessel array comprises a plurality of vessel compartments, each comprising a compartment vertical length axis that is substantially coincident with the length axis of the elongated vessel, such that the plurality of elongated vessels are arranged in the vessel array in a substantially vertical position;
(b) an array of magnets, wherein each magnet is operably associated with a vessel compartment of the array, each magnet comprising a plane outer surface in communication with the outer surface of the side wall of the vessel; and
(c) a transport means comprising a belt drive, wherein the array of magnets is rotatably mounted to the belt drive and the belt drive is configured to move the array of magnets along a motion path positioned between first and second predetermined positions of said apparatus, wherein the first predetermined position is substantially aligned with the central part of the lower portion of the elongated vessel and the second predetermined position is substantially aligned with the bottom of the elongated vessel, and the motion path is substantially parallel with the length axis of the elongated vessel, and said belt drive is configured to move said array of magnets along said motion path such that over at least a portion of said motion path, said plane outer surface of said magnet is in contact with at least a portion of said outer surface of said side wall of said vessel.

2. The apparatus of claim 1 wherein the belt drive further comprises one or more tooth wheels in operable communication with a tension pulley.

3. The apparatus of claim 1 wherein the transport means is configured to move the array of magnets downwards from the first to the second predetermined positions.

4. The apparatus of claim 1 wherein said vessel array comprises an upper part having a substantially cuboid shape.

5. The apparatus according to claim 1 , wherein said transport means further comprises a magnet holder having a cavity adapted to receive a portion of the magnet upon rotatable engagement of the transport means along said motion path.

6. The apparatus according to claim 5 , wherein the magnet holder further comprises one or more springs positioned in the cavity and capable of exerting a predetermined force on said magnet and said outer surface of the side wall when said magnet is moved over said portion of said motion path.

7. The apparatus of claim 5 wherein the magnet holder comprises a body comprising a plurality of individual magnet holders.

8. The apparatus of claim 7 wherein the plurality of individual magnet holders are rigidly connected in the body.

9. The apparatus according to claim 1 , wherein the motion path is a closed motion path.

10. The apparatus according to claim 1 , wherein the magnet has a magnetic axis that forms an angle of about 45 degrees with the plane outer surface of the magnet.

Assignees

• Roche Molecular Systems Inc

Inventors

• Holenstein Tobias
• Schneebeli Rolf
• Belz Renato

Classifications

• B01L9/543

  for disposable pipette tips, e.g. racks or cassettes · CPC title

• B03C1/288

  disposed at the outer circumference of a recipient · CPC title

• B03C1/12

  with magnets moving during operation; with movable pole pieces · CPC title

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title

• B01L3/5085 Primary

  for multiple samples, e.g. microtitration plates · CPC title