Agent for preventing bacterial wilt disease, and method for preventing bacterial wilt disease

Patent metadata

Field	Value
Publication number	US-9380786-B2
Application number	US-201214113802-A
Country	US
Kind code	B2
Filing date	Apr 27, 2012
Priority date	Apr 28, 2011
Publication date	Jul 5, 2016
Grant date	Jul 5, 2016

Abstract

Provided are: an agent for preventing bacterial wilt disease, which agent can prevent the development of bacterial wilt disease caused by various different bacterial wilt disease strains, that is, the development of bacterial wilt disease in various different plant varieties; and a method for preventing bacterial wilt disease utilizing the agent for preventing bacterial wilt disease. The agent for preventing bacterial wilt disease comprises as an active ingredient a wilt bacterium ( Ralstonia solanacearum ) infected with the φRSM1-type filamentous phage and/or the φRSM3-type filamentous phage. The method for preventing bacterial wilt disease comprises the step of inoculating the agent for preventing bacterial wilt disease to a plant. The plant is preferably any one of tomato, potato, green pepper, eggplant, tobacco, capsicum, Japanese basil, Japanese radish, strawberry, banana, marguerite, chrysanthemum and sunflower.

First claim

The invention claimed is:

1. A method for preventing bacterial wilt disease, comprising inoculating with an agent for preventing bacterial wilt disease comprising as an effective component a wilt bacterium ( Ralstonia solanacearum ) infected with a φRSM1 filamentous phage and a wilt bacterium ( Ralstonia solanacearum ) infected with a φRSM3 filamentous phage into a plant that needs prevention of bacterial wilt disease caused by wilt disease bacterium having pathogenicity, thereby preventing bacterial wilt disease in plants later exposed to a pathogenic wilt disease bacterium for at least two months.

2. The method for preventing bacterial wilt disease according to claim 1 , wherein the plant is any one of tomato, potato, green pepper, eggplant, tobacco, capsicum, Japanese basil, Japanese radish, strawberry, banana, marguerite, chrysanthemum and sunflower.

3. The method for preventing bacterial wilt disease according to claim 2 , wherein the plant is any one of tomato, potato, green pepper, eggplant and tobacco.

4. The method for preventing bacterial wilt disease according to claim 1 , wherein the method for preventing bacterial wilt disease comprises inoculating a plant with a wilt bacterium ( Ralstonia solanacearum ) infected with the φRSM1 filamentous phage and a wilt bacterium ( Ralstonia solanacearum ) infected with the φRSM3 filamentous phage in an amount of 10 5 to 10 8 cells/g plant body weight.

5. The method for preventing bacterial wilt disease according to claim 4 , wherein the method for preventing bacterial wilt disease comprises inoculating a plant with a wilt bacterium ( Ralstonia solanacearum ) infected with the φRSM1 filamentous phage and a wilt bacterium ( Ralstonia solanacearum ) infected with the φRSM3 filamentous phage in an amount of 10 6 to 10 7 cells/g plant body weight.

6. The method for preventing bacterial wilt disease according to claim 1 , wherein the agent for preventing bacterial wilt disease is inoculated into the stem of the plant.

7. The method for preventing bacterial wilt disease according to claim 6 , wherein the agent for preventing bacterial wilt disease is inoculated into the stem of the plant at a site 1 to 4 cm distant from the soil.

8. The method for preventing bacterial wilt disease according to claim 1 , wherein the agent for preventing bacterial wilt disease is inoculated 2 to 4 weeks after germination of the plant.

9. The method for preventing bacterial wilt disease according to claim 1 , wherein the wilt bacterium ( Ralstonia solanacearum ) strain infected with the φRSM1 filamentous phage is any one of M4S, Ps29, Ps65 and Ps74.

10. The method for preventing bacterial wilt disease according to claim 1 , wherein the wilt bacterium ( Ralstonia solanacearum ) strain infected with the φRSM3 filamentous phage is any one of C319, Ps72 and Ps74.

Assignees

• Yamada Takashi
• Fujie Makoto
• Kawasaki Takeru
• Univ Hiroshima

Inventors

• Yamada Takashi
• Fujie Makoto
• Kawasaki Takeru

Classifications

• A01N63/00 Primary

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• C12N2795/14131

  Uses of virus other than therapeutic or vaccine, e.g. disinfectant · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12N7/00

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• C12N2795/14121

  Viruses as such, e.g. new isolates, mutants or their genomic sequences · CPC title