Antibiotic-free plasmids
US-9217153-B2 · Dec 22, 2015 · US
Methods of modifying N-termini of a peptide or protein using transferases
US9376700B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9376700-B2 |
| Application number | US-201314406864-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 13, 2013 |
| Priority date | Jun 14, 2012 |
| Publication date | Jun 28, 2016 |
| Grant date | Jun 28, 2016 |
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- Title
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Abstract
Official abstract text for this publication.
The invention includes a selective method of modifying the N-terminus of a protein using an aminoacyl tRNA transferase. In certain embodiments, the method comprises contacting a solution of the protein or peptide with a transferase and a derivative of a molecule, whereby the N-terminus of the protein or peptide is derivatized with the molecule.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of derivatizing the N-terminus of a protein or peptide with an amino acid, the method comprising contacting in a solution the protein or peptide with an aminoacyl tRNA transferase (AaT), or a mutant thereof, and an adenosine ester of the amino acid, whereby the N-terminus of the protein or peptide is derivatized with the amino acid. 2. The method of claim 1 , wherein the method is carried out under conditions that do not significantly denature the protein or peptide. 3. The method of claim 1 , wherein the solution is substantially free of a synthetase. 4. The method of claim 3 , wherein the synthetase comprises aminoacyl tRNA synthetase. 5. The method of claim 1 , wherein the amino acid comprises a compound of Formula wherein Xaa is selected from the group consisting of: a salt thereof and any combinations thereof. 6. The method of claim 1 , wherein the amino acid is selected from the group consisting of cysteine, homocysteine, selenocysteine, selenohomocysteine, a derivative thereof and any combinations thereof. 7. The method of claim 1 , wherein the transferase comprises E. coli AaT, V. vulnificus BpT, a mutant thereof and any combinations thereof. 8. The method of claim 1 , wherein the amino acid comprises a detectable label. 9. The method of claim 8 , wherein the detectable label is selected from the group consisting of a radioisotope, stable isotope, fluorophore, electron dense metals, biotin, DNA, RNA, antibody epitope, and any combinations thereof.
Assignees
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Classifications
attached in position 6, e.g. adenine · CPC title
Mononitriles · CPC title
- C12P21/00Primary
Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title
not hydrogenated in the hetero ring · CPC title
Labelling of peptides · CPC title
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- What does patent US9376700B2 cover?
- The invention includes a selective method of modifying the N-terminus of a protein using an aminoacyl tRNA transferase. In certain embodiments, the method comprises contacting a solution of the protein or peptide with a transferase and a derivative of a molecule, whereby the N-terminus of the protein or peptide is derivatized with the molecule.
- Who is the assignee on this patent?
- Univ Pennsylvania
- What technology area does this patent fall under?
- Primary CPC classification C12P21/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 28 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).