Method for preparing optically active amino acid using cosubstrate shuttling of transaminase

Patent metadata

Field	Value
Publication number	US-9376694-B2
Application number	US-201314347174-A
Country	US
Kind code	B2
Filing date	Jul 8, 2013
Priority date	Jul 6, 2012
Publication date	Jun 28, 2016
Grant date	Jun 28, 2016

Abstract

The present disclosure relates to a method for preparing an optically active amino acid using cosubstrate shuttling of transaminase. The method includes coupling a reaction of converting a keto acid to an amino acid by α-transaminase and a reaction of transferring an amino group of an amine substrate by ω-transaminase (TA) using an amino acid cosubstrate. The present disclosure allows production of various optically active amino acids with high purity and high efficiency by solving the low equilibrium constant problem of transaminase and is applicable to production of various optically active amino acids in industrial scale. Since the present disclosure allows easy production of various unnatural amino acids having high reactivity and stability, which are used as pharmaceutical precursors, it can be usefully employed in preparation of pharmaceuticals, food additives and various animal feeds.

First claim

The invention claimed is:

1. A method for preparing an optically active amino acid by a cascade reaction, comprising:
(A) converting a keto acid to an amino acid substrate and an amino acid cosubstrate by alpha transaminase wherein the amino acid cosubstrate is converted into a keto acid of the amino acid substrate; and
(B) transferring an amino group of an amine substrate to the keto acid of the amino acid cosubstrate by ω-transaminase (ω-TA), and generating the amino acid cosubstrate, wherein the amine substrate is isopropylamine and the cascade reaction produces an optically active amino acid.

2. The method for preparing an optically active amino acid according to claim 1 , wherein the amino acid cosubstrate is an amino acid showing reactivity for both α-transaminase and ω-transaminase (ω-TA).

3. The method for preparing an optically active amino acid according to claim 1 , wherein the keto acid is selected from pyruvate, 2-oxobutyrate, 2-(3-hydroxy-1-adamantyl)-2-oxoethanoic acid, trimethylpyruvate, 3-methyl-2-oxobutyrate, 3-methyl-2oxopentanoic acid, 4-methyl-2-oxopentanoic acid, 2-oxopentanoic acid, 2-oxohexanoic acid, 2-oxooctanoic acid, fluoropyruvate, hydroxypyruvate, mercaptopyruvate, oxaloacetate, ketoglutarate, phenylglyoxylate, phenylpyruvate, 4-hydroxyphenylglyoxylate, 4-dimethyl-2oxopentanoic acid, 3-dimethyl-2-oxopentanoic acid, 3-ethyl-3-methyl-2-oxopentanoic acid and 5-dimethyl-2-oxohexanoic acid.

4. The method for preparing an optically active amino acid according to claim 1 , wherein the amine substrate is selected from benzylamine, methylbenzylamine, ethylbenzylamine, isopropylamine, 2-butylamine, 1-aminoindane, cyclopropylethylamine, 2-aminopentane, 3-methyl-2-butylamine, 1,3-dimethylbutylamine, 2-aminooctane, 1-methoxy-2-propylamine, 2-aminohexane, p-fluoromethylbenzylamine, mexiletine and 1-methyl-3-phenylpropylamine.

5. The method for preparing an optically active amino acid according to claim 1 , wherein the optically active amino acid is selected from alanine, homoalanine, norvaline, norleucine, 2-aminocaprylic acid, valine, leucine, isoleucine, tert-leucine, fluoroalanine, serine, cysteine, aspartate, glutamate, phenylglycine, phenylalanine, 4-hydroxyphenylalanine, 3-hydroxyadamantylglycine, neopentylglycine, 3-dimethyl-2-aminopentanoic acid, 3-ethyl-3-methyl-2-aminopentanoic acid and 5-dimethyl-2-aminohexanoic acid in L - or D -form.

6. The method for preparing an optically active amino acid according to claim 1 , wherein the α-transaminase is branched-chain transaminase (BCTA), D -amino-acid transaminase (DATA), aromatic-amino-acid transaminase (AroTA), aspartate transaminase (AspTA) or alanine transaminase (ATA) and the ω-transaminase is one isolated from Paracoccus denitrificans, Ochrobactrum anthropic or Arthrobacter species.

7. The method for preparing an optically active amino acid according to claim 1 , wherein, in the cascade reaction, the concentration of the amino acid cosubstrate is 0.1-20% of the concentration of the keto acid substrate.

8. The method for preparing an optically active amino acid according to claim 1 , wherein the cascade reaction is conducted by further adding an organic solvent if the reactivity of the ω-transaminase is inhibited by a ketone or an aldehyde.

Assignees

• Univ Yonsei Iacf

Inventors

• Shin Jong-Shik
• Park Eul-Soo

Classifications

• C12P13/14 Primary

  Glutamic acid; Glutamine · CPC title

• C12N9/1096

  transferring nitrogenous groups (2.6) · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12P13/06 Primary

  Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title

• C12P13/08

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title