System for processing contents of a receptacle to detect an optical signal emitted by the contents

Patent metadata

Field	Value
Publication number	US-9372156-B2
Application number	US-201113031889-A
Country	US
Kind code	B2
Filing date	Feb 22, 2011
Priority date	Mar 10, 2005
Publication date	Jun 21, 2016
Grant date	Jun 21, 2016

Abstract

A system is configured to process the contents of one or more receptacles to detect an optical signal emitted by the contents of each receptacle. Each receptacle is held within a receptacle station of a movable receptacle carrier disposed within a temperature-controlled chamber of an incubator. At least one signal detector is configured to detect an optical signal emitted by the contents of a receptacle as the receptacle carrier moves each receptacle into an operative position with respect to the signal detector. A magnet holder is attached to the receptacle carrier and holds one or more magnets adjacent to an associated receptacle station so as to expose the contents of each receptacle carried in the receptacle station to a magnetic field when the receptacle is in the operative position with respect to the signal detector.

First claim

The invention claimed is:

1. A system for processing the contents of one or more receptacles to detect an optical signal, if any, emitted by the contents of each receptacle, the system comprising:
an incubator including a temperature-controlled chamber configured to receive one or more receptacles;
at least one signal detector configured to detect an optical signal, if any, emitted by the contents of a receptacle disposed within said chamber in an operative position with respect to the signal detector;
a receptacle carrier disposed within said chamber and comprising one or more receptacle stations, each receptacle station being configured to receive and hold one or more receptacles; and
at least one magnet holder attached to said receptacle carrier and holding one or more magnets in a fixed position adjacent to an associated receptacle carrier station so as to expose the contents of each of one or more receptacles carried in the associated receptacle station to a magnetic field when each of the receptacles is in the operative position with respect to said at least one signal detector, and wherein said receptacle carrier, including said one or more receptacle stations, said magnet holder, and said one or more magnets, is automatically moveable relative to said at least one signal detector.

2. The system of claim 1 , wherein an individual one of said magnets is disposed so as to be adjacent to each of the receptacles that is in the operative position with respect to each signal detector.

3. The system of claim 1 , wherein said receptacle carrier comprises a carousel that is rotatable about an axis of rotation and which comprises a plurality of said receptacle stations arranged around a circumference of said carousel, and wherein said magnet holder associated with each receptacle station is a magnetic divider attached to said carousel between the associated receptacle station and an adjacent receptacle station.

4. The system of claim 3 , wherein said magnetic divider comprises:
a magnet block attached to said carrousel, said magnet block having formed therein an opening for receiving each magnet carried on said magnetic divider; and
a retainer plate secured to said magnet block and configured to retain each magnet within its opening.

5. The system of claim 4 , wherein each opening formed in said magnet block is circular and each magnet is disc-shaped.

6. The system of claim 3 , wherein each magnetic divider comprises a divider wall that is oriented generally radially with respect to the axis of rotation, and the divider walls of said magnetic dividers are positioned at angularly-spaced positions about the axis of rotation.

7. The system of claim 1 , wherein each magnet comprises neodymium-iron-boron.

8. The system of claim 1 , wherein said incubator comprises a housing at least partially enclosing said chamber with one or more doors formed in said housing through which receptacles can be moved into and out of said chamber.

9. The system of claim 8 , wherein said at least one signal detector is located outside said housing, and wherein said housing includes an opening through which each said signal detector can detect an optical signal, if any, emitted by the contents of a receptacle located inside said housing in the operative position with respect to the signal detector.

10. The system of claim 1 , wherein said at least one signal detector comprises a fluorometer configured to detect a fluorescent signal, if any, emitted by the contents of a receptacle operatively positioned with respect to said fluorometer.

11. The system of claim 1 , further comprising a controller programmed so as to determine the presence or amount of a target nucleic acid within the contents of each receptacle based on an optical signal detected by said at least one signal detector.

12. The system of claim 1 , further comprising a separation station having movable magnets for selectively exposing the contents of a receptacle within said separation station to a magnetic field, thereby isolating magnetically-responsive solid supports within the receptacle.

13. The system of claim 12 , wherein the separation station comprises a fluid aspirator configured to aspirate the fluid contents of the receptacle within said separation station.

14. The system of claim 13 , wherein the separation station further comprises a fluid dispenser configured to dispense a wash solution into the receptacle within said separation station.

15. The system of claim 14 , wherein the separation station further comprises a mixing device configured to agitate the contents of the receptacle within said separation station.

16. The system of claim 12 , further comprising an automated receptacle transport device constructed and arranged to move receptacles from said separation station to said incubator.

17. A system for processing the contents of a plurality of receptacles to detect an optical signal, if any, emitted by the contents of each receptacle, the system comprising:
an incubator including a temperature-controlled chamber configured to receive a plurality of receptacles;
at least one signal detector configured to detect an optical signal, if any, emitted by the contents of a receptacle disposed within said chamber in an operative position with respect to the signal detector;
a receptacle carrier disposed within said chamber and comprising one or more receptacle stations, wherein each receptacle station is configured to hold a plurality of receptacles arranged in an aligned configuration; and
at least one magnet holder attached to the receptacle carrier and holding a plurality of magnets in a fixed position adjacent to an associated receptacle station so as to expose the contents of each of the receptacles carried in the associated receptacle station to a magnetic field of at least one of the magnets when the receptacles are in the operative position with respect to said signal detector, and wherein said receptacle carrier, including said one or more receptacle stations, said magnet holder, and said magnets, is automatically moveable relative to said at least one signal detector.

18. The system of claim 1 , wherein the at least one signal detector comprises two or more signal detectors configured to detect an optical signal, if any, emitted by the contents of a receptacle disposed within said chamber in an operative position with respect to any of the signal detectors, wherein each signal detector is configured to detect an optical signal that is the same as the optical signal detected by at least one other signal detector or each signal detector is configured to detect an optical signal that is different from the optical signal detected by each other signal detector.

19. The system of claim 17 , wherein the at least one signal detector comprises two or more signal detectors configured to detect an optical signal, if any, emitted by the contents of a receptacle disposed within said chamber in an operative position with respect to any of the signal detectors, wherein each signal detector is configured to detect an optical signal that is the same as the optical signal detected by at least one other signal detector or each signal detector is configured to detect an optical signal that is different from the optical signal detected by each other signal detector.

20. The system of claim 17 , wherein said receptacle carrier comprises a carousel that is rotatable about an axis of rotation and which comprises a plurality of said receptacle stations arranged around a circumference of said c

Assignees

• Knight Byron J
• Gen Probe Inc

Inventors

• Knight Byron J

Classifications

• G01N2021/6419

  Excitation at two or more wavelengths · CPC title

• G01N35/0099

  comprising robots or similar manipulators (robots per se B25J) · CPC title

• G01N21/6428 Primary

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N2021/6421

  Measuring at two or more wavelengths · CPC title

• C12Q1/6851

  Quantitative amplification · CPC title