Photosynthetic microorganisms expressing thermostable lipase
US-9303264-B2 · Apr 5, 2016 · US
Reagents for lysis of bacterial cells
US9371509B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9371509-B2 |
| Application number | US-85580310-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 13, 2010 |
| Priority date | Sep 2, 2009 |
| Publication date | Jun 21, 2016 |
| Grant date | Jun 21, 2016 |
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Abstract
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The invention provides a composition containing an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase. The ionic compound is selected from the group consisting of 1-butyl-3-methyl-imidazolium-thiocyanate, 1-butyl-3-methyl-imidazolium-2(2-methoxy-ethoxy)ethylsulfate, 1-methyl-1-[4-(3-methyl-3H-imidazol-1-ium)-but-1-yl]-3H-imidazolium-di(toluylsulfate), and 1-butyl-3-methyl-imidazolium-octylsulfate. The compositions of the invention are advantageously used for preparing lysates of biological cells, particularly bacterial cells.
First claim
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What is claimed is: 1. A composition comprising an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase at a concentration of about 560 mM or higher, wherein the ionic compound is 1-butyl-3-methyl-imidazolium-thiocyanate and, with the proviso that the composition does not include a detergent or chaotropic agent. 2. The composition according to claim 1 , further comprising a buffer salt capable of buffering at an acidic pH. 3. The composition according to claim 1 , wherein the composition has an acidic pH. 4. The composition according to claim 1 , further comprising a solid phase with a silica surface. 5. The composition according to claim 4 , wherein the solid phase comprises magnetically attractable particles. 6. A composition comprising an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase at a concentration of at least about 560 mM, wherein the ionic compound is 1-butyl-3-methyl-imidazolium-thiocyanate and the bacterial cells are one of Gram-negative cells, Gram-positive cells, and a mixture thereof, with the proviso that the composition does not include a detergent or chaotropic agent. 7. The composition according to claim 6 , further comprising a buffer salt capable of buffering at an acidic pH. 8. The composition according to claim 6 , wherein the composition has an acidic pH. 9. The composition according to claim 6 , further comprising a solid phase with a silica surface. 10. The composition according to claim 9 , wherein the solid phase comprises magnetically attractable particles.
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- What does patent US9371509B2 cover?
- The invention provides a composition containing an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase. The ionic compound is selected from the group consisting of 1-butyl-3-methyl-imidazolium-thiocyanate, 1-butyl-3-methyl-imidazolium-2(2-methoxy-ethoxy)ethylsulfate, 1-methyl-1-[4-(3-methyl-3H-imidazol-1-ium)-but-1-yl]-3H-imidazolium-di(toluylsulfate), and…
- Who is the assignee on this patent?
- Birkner Christian, Eltz Herbert Von Der, Soukupova Monika, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N1/06. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 21 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).