Amplification system with spatial separation

Patent metadata

Field	Value
Publication number	US-9365895-B2
Application number	US-201313962824-A
Country	US
Kind code	B2
Filing date	Aug 8, 2013
Priority date	Dec 10, 2009
Publication date	Jun 14, 2016
Grant date	Jun 14, 2016

Abstract

An automated nucleic acid analysis method and analytical system are described comprising separate modules, wherein the air flow of any one of said modules is controlled and wherein at least the air flow between the module for isolation and purification of the analyte and the module for analysis of the analyte are separated.

First claim

What is claimed is:

1. An automated analytical apparatus for processing an analyte, comprising:
a sample module comprising a sample dispensing station configured to dispense liquid samples comprising an analyte from a sample vessel to a processing vessel containing magnetic particles with pipette tips held in a rack;
a processing module comprising a separation device for isolating and purifying said analyte, the separation device comprising at least one movable magnet arranged to interact with said processing vessel, wherein said processing module has a first air flow and comprises an air pressure;
an analytical module for analyzing said analyte contained in a reaction vessel, wherein said analytical module has a second air flow;
a transfer system for transferring said analyte from the processing vessel to the reaction vessel and transferring the reaction vessel from the processing module to the analytical module;
wherein, the first air flow and the second air flow are separated from each other, and wherein said sample module has an air flow which is separate from said first air flow of said processing module and said second air flow of said analytical module, and wherein said sample module comprises an air pressure that is the same as the air pressure of said processing module.

2. The automated analytical apparatus of claim 1 , wherein said analytical module comprises a second air pressure that is lower than said air pressure of said sample module and said air pressure of said processing module.

3. The automated analytical apparatus of claim 1 , wherein an air-lock is located between said processing module and said analytical module.

4. The automated analytical apparatus of claim 3 , wherein said transfer system comprises a first handler for transferring said reaction vessel from said processing module to said air-lock, and a second handler for transferring said reaction vessel from said air-lock to said analytical module.

5. The automated analytical apparatus of claim 1 , wherein said apparatus comprises separation walls located among said sample module, said processing module and said analytical module.

6. The automated analytical apparatus of claim 1 , wherein said sample module comprises a filter for filtration of air flowing in said sample module.

7. The automated analytical apparatus of claim 1 , additionally comprising gaskets in an upper housing of said processing module.

Assignees

• Roche Molecular Systems Inc

Inventors

• Bernet Roland
• Gisler Andreas
• Kaeppeli Marcel
• Ochsenbein Raymond
• Schlaubitz Thomas

Classifications

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title

• B01L3/0275

  Interchangeable or disposable dispensing tips · CPC title

• G01N35/0099

  comprising robots or similar manipulators (robots per se B25J) · CPC title

• B01L2200/028

  Modular arrangements · CPC title

• B01L9/543

  for disposable pipette tips, e.g. racks or cassettes · CPC title