Method for producing chemical by continuous fermentation

Patent metadata

Field	Value
Publication number	US-9365876-B2
Application number	US-201113992443-A
Country	US
Kind code	B2
Filing date	Dec 8, 2011
Priority date	Dec 9, 2010
Publication date	Jun 14, 2016
Grant date	Jun 14, 2016

Abstract

A method produces a chemical through continuous fermentation including: (a) culturing a cell in a culture medium in a fermentor to ferment a feedstock to produce a chemical; (b) conducting filtration of the culture medium with a separation membrane module; (c) separating a permeate containing the chemical from the culture medium while retaining a non-permeated liquid in the fermentor, and (d) supplying a gas from at least one of a lower portion of the separation membrane module and a pipe communicating between the fermentor and the separation membrane module to adjust a gas linear velocity in the separation membrane module to 0.15 cm/s to 70 cm/s while supplying the separation membrane module with a liquid.

First claim

The invention claimed is:

1. A method of producing a chemical through continuous fermentation comprising:
(a) culturing a cell in a culture medium in a fermentor to ferment a feedstock to produce a chemical;
(b) conducting filtration of the culture medium with a separation membrane module;
(c) separating a permeate containing the chemical from the culture medium while retaining a non-permeated liquid in the fermentor, and
(d) supplying a gas from at least one of a lower portion of the separation membrane module and a pipe communicating between the fermentor and the separation membrane module to adjust a gas linear velocity in the separation membrane module to 0.15 cm/s to 70 cm/s while supplying the separation membrane module with the culture medium.

2. The method according to claim 1 , wherein in (d), the gas contains oxygen.

3. The method according to claim 2 , further comprising, in addition to (d), (e) supplying the fermentor with a gas, wherein:
the gas is supplied in (d) intermittently, and
when the gas is not supplied in (d), a supply rate of the gas in (e) is increased compared with that when the gas is supplied in (d).

4. The method according to claim 1 , wherein the filtration in (b) is conducted intermittently.

5. The method according to claim 1 , wherein the cell is a microorganism.

6. The method according to claim 5 , wherein the microorganism is a microorganism belonging to any of the Genus Escherichia , the Genus Providencia , the Genus Corynebacterium , the Genus Brevibacterium , and the Genus Serratia.

7. The method according to claim 6 , wherein the microorganism is any of Escherichia coli, Providencia rettgeri, Corynebacterium glutamicum, Brevibacterium flavum, Brevibacterium lactofermentum , and Serratia marcescens.

8. The method according to claim 1 , wherein the cell is a yeast.

9. The method according to claim 1 , wherein the chemical is an amino acid.

10. The method according to claim 9 , wherein the amino acid is L-threonine, L-lysine, L-glutamic acid, L-tryptophan, L-isoleucine, L-glutamine, L-arginine, L-alanine, L-histidine, L-proline, L-phenylalanine, L-aspartic acid, L-tyrosine, L-methionine, L-serine, L-valine, or L-leucine.

11. The method according to claim 1 , wherein the chemical is an organic acid.

12. The method according to claim 11 , wherein the chemical is lactic acid.

13. The method according to claim 1 , wherein the chemical is cadaverine.

Assignees

• Kanamori Satoko
• Cheon Jihoon
• Mimitsuka Takashi
• Takeuchi Norihiro
• Nishida Makoto
• Tanaka Yuji
• Toray Industries

Inventors

• Kanamori Satoko
• Cheon Jihoon
• Mimitsuka Takashi
• Takeuchi Norihiro
• Nishida Makoto
• Tanaka Yuji

Classifications

• C12M47/10

  Separation or concentration of fermentation products (bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02) · CPC title

• C12P7/56

  Lactic acid · CPC title

• C12M29/02

  Percolation · CPC title

• C12M41/40

  of pressure · CPC title

• C12P13/08 Primary

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title