Methods and compositions related to cyclic peptide synthesis

Patent metadata

Field	Value
Publication number	US-9365622-B2
Application number	US-28137307-A
Country	US
Kind code	B2
Filing date	Mar 1, 2007
Priority date	Mar 1, 2006
Publication date	Jun 14, 2016
Grant date	Jun 14, 2016

Abstract

Disclosed are compositions and methods for cyclization of polymers such as peptides.

First claim

What is claimed is:

1. A peptide comprising an amino acid segment comprising:
the amino acid sequence of GLEAS(N 1 )AYDGVEPS(N 2 )AYDGE,
an amino acid sequence at least about 90% identical to the amino acid sequence of GLEAS(N 1 )AYDGVEPS(N 2 )AYDGE, or
the amino acid sequence of GLEAS(N 1 )AYDGVEPS(N 2 )AYDGE having one or more conservative amino acid substitutions;
wherein N 1 and/or N 2 comprises an amino acid sequence,

wherein N 1 or N 2 do not comprise one or more of the amino acids A, C, I, F, L, P, S, T, or V.

2. The peptide of claim 1 , wherein the amino acid sequence of N 1 comprises less than 100 amino acids.

3. The peptide of claim 1 , wherein the amino acid sequence of N 1 comprises less than 50 amino acids.

4. The peptide of claim 1 , wherein the amino acid sequence of N 1 comprises less than 20 amino acids.

5. The peptide of claim 1 , wherein the amino acid sequence of N 1 comprises less than 10 amino acids.

6. The peptide of claim 1 , wherein the amino acid sequence of N 2 comprises less than 100 amino acids.

7. The peptide of claim 1 , wherein the amino acid sequence of N 2 comprises less than 50 amino acids.

8. The peptide of claim 1 , wherein the amino acid sequence of N 2 comprises less than 20 amino acids.

9. The peptide of claim 1 , wherein the amino acid sequence of N 2 comprises less than 10 amino acids.

10. A peptide comprising an amino acid segment comprising:
the amino acid sequence of GLEAS(N 1 )AYDGVEPS(N 2 )AYDGE,
an amino acid sequence at least about 90% identical to the amino acid sequence of GLEAS(N 1 )AYDGVEPS(N 2 )AYDGE, or
the amino acid sequence of GLEAS(N 1 )AYDGVEPS(N 2 )AYDGE having one or more conservative amino acid substitutions;
wherein N 1 and N 2 comprises an amino acid sequence,
wherein N 1 and N 2 do not comprise one or more of the amino acids A, C, I, F, L, P, S, T, or V.

11. The peptide of claim 10 , wherein the amino acid sequence of N 1 comprises less than 100 amino acids.

12. The peptide of claim 10 , wherein the amino acid sequence of N 1 comprises less than 50 amino acids.

13. The peptide of claim 11 , wherein the amino acid sequence of N 1 comprises less than 20 amino acids.

14. The peptide of claim 10 , wherein the amino acid sequence of N 1 comprises less than 10 amino acids.

15. The peptide of claim 10 , wherein the amino acid sequence of N 2 comprises less than 100 amino acids.

16. The peptide of claim 10 , wherein the amino acid sequence of N 2 comprises less than 50 amino acids.

17. The peptide of claim 10 , wherein the amino acid sequence of N 2 comprises less than 20 amino acids.

18. The peptide of claim 10 , wherein the amino acid sequence of N 2 comprises less than 10 amino acids.

19. The peptide of claim 10 , wherein one or more of the amino acids is functionalized.

Assignees

• Schmidt Eric W
• Hathaway Brian
• Nelson James T
• Donia Mohamed S
• Univ Utah Res Found

Inventors

• Schmidt Eric W
• Hathaway Brian
• Nelson James T
• Donia Mohamed S

Classifications

• G01N2500/04

  Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A) · CPC title

• G01N33/6845

  Methods of identifying protein-protein interactions in protein mixtures · CPC title

• C12N15/74

  Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora · CPC title

• C07K14/00 Primary

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C07K7/64 Primary

  Cyclic peptides containing only normal peptide links · CPC title