Micro-fluidic modules on a chip for diagnostic applications

Patent metadata

Field	Value
Publication number	US-9364833-B2
Application number	US-201313967882-A
Country	US
Kind code	B2
Filing date	Aug 15, 2013
Priority date	Aug 17, 2012
Publication date	Jun 14, 2016
Grant date	Jun 14, 2016

Abstract

A chip having a substrate for amplifying genetic material includes a substrate heater having a plurality of substrate heating resistors. The heating resistors define a first temperature zone for maintaining a first temperature, a second temperature zone for maintaining a second temperature below the first temperature, and a third temperature zone for maintaining a third temperature between the first and second temperatures, on the substrate. A flow path formed on the substrate allows conveying of reactions to the first temperature zone, the third temperature zone, and the second temperature zone so as to undergo denaturing, annealing, and extension, respectively, according to a polymerase chain reaction process, for at least one cycle.

First claim

The invention claimed is:

1. A chip for amplifying genetic material, comprising:
a substrate having a top surface and a backside defining a thickness thereof;
at least two trenches through an entirety of the thickness of the substrate, each of the trenches being isolated from one another;
a substrate heater having a plurality of substrate heating resistors along a length of the substrate that define a first temperature zone for maintaining a first temperature, a second temperature zone for maintaining a second temperature below the first temperature, and a third temperature zone for maintaining a third temperature between the first and second temperatures, on the substrate, wherein one of the at least two trenches exists between the first and third temperature zones and the other of the at least two trenches exists between the second and third temperature zones thereby thermally isolating the substrate heating resistors in distinct temperature regions along the length of the substrate; and
a flow path formed on the substrate between a fluid inlet and outlet at ends of the length of the substrate for conveying reactions to the first temperature zone, the third temperature zone, and the second temperature zone to allow the reactions to undergo denaturing, annealing, and extension, respectively, according to a polymerase chain reaction process for at least one cycle, wherein the flow path extends in a flow feature layer on the substrate under a cover defining a serpentine channel having a plurality of cycles along an extent of the serpentine channel, wherein the cycles pass through each of the first, second and third temperature zones but wherein none of the substrate heating resistors are disposed in the cycles along the extent of the serpentine channel that passes above the at least two trenches.

2. The chip of claim 1 , further comprising one or more pumps disposed along the flow path for compelling the reactions to move along the flow path.

3. The chip of claim 1 , wherein the flow path is formed by a flow feature layer disposed above the substrate heater.

4. The chip of claim 1 , wherein the first temperature is above 90° C.

5. The chip of claim 1 , wherein the second temperature is between about 70° C. and about 75° C.

6. The chip of claim 1 , wherein the third temperature is between about 50° C. and about 65° C.

Assignees

• Lexmark Int Inc

Inventors

• Bergstedt Steven W
• Demoor Stephen J
• Guan Yimin

Classifications

• B01L2200/147

  Employing temperature sensors · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title

• B01L7/52 Primary

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L2400/0442

  thermal energy, e.g. vaporisation, bubble jet · CPC title