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US9360487B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9360487-B2 |
| Application number | US-201414223524-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 24, 2014 |
| Priority date | Sep 22, 2011 |
| Publication date | Jun 7, 2016 |
| Grant date | Jun 7, 2016 |
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Abstract
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The current disclosure provides specific peptides, and derived ionization characteristics of the peptides from the estrogen receptor (ER), progesterone receptor (PR), and/or antigen Ki67 (Ki67) proteins that are particularly advantageous for quantifying the ER, PR, and/or Ki67 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring/Multiple Reaction Monitoring (SRM/MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein the biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from a biological sample using the Liquid Tissue™ reagents and protocol, and the ER, PR, and/or Ki67 proteins are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described for one or more of the ER, PR, and/or Ki67 proteins. These peptides can be quantitated if they reside in a modified or in an unmodified form. An example of a modified form of an ER, PR, and/or Ki67 peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for measuring the amount of the human Estrogen Receptor (ER), protein in a biological sample of formalin-fixed tissue, comprising detecting and/or quantifying the amount of an ER fragment peptide in a protein digest prepared from said biological sample using mass spectrometry; and calculating the amount of ER protein in said sample; wherein said ER fragment peptide is SEQ ID NO:2 and wherein the amount is a relative amount or an absolute amoun…
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Physics · mapped topic
- G01N33/6893Primary
Physics · mapped topic
Physics · mapped topic
- G01N33/6848Primary
Physics · mapped topic
Physics · mapped topic
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- What does patent US9360487B2 cover?
- The current disclosure provides specific peptides, and derived ionization characteristics of the peptides from the estrogen receptor (ER), progesterone receptor (PR), and/or antigen Ki67 (Ki67) proteins that are particularly advantageous for quantifying the ER, PR, and/or Ki67 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring/…
- Who is the assignee on this patent?
- Expression Pathology Inc
- What technology area does this patent fall under?
- Primary CPC classification G01N33/6893. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Jun 07 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).