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Method and system for accurate alignment and registration of array for DNA sequencing
US9359641B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9359641-B2 |
| Application number | US-201414309049-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 19, 2014 |
| Priority date | Apr 30, 2010 |
| Publication date | Jun 7, 2016 |
| Grant date | Jun 7, 2016 |
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Abstract
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In a genome sequencing system and methodology, a protocol is provided to achieve precise alignment and accurate registration of an image of a planar array of nanoballs subject to optical analysis. Precise alignment correcting for fractional offsets is achieved by correcting for errors in subperiod x-y offset, scale and rotation by use of minimization techniques and Moiré averaging. In Moiré averaging, magnification is intentionally set so that the pixel period of the imaging element is a noninteger multiple of the site period. Accurate registration is achieved by providing for pre-defined pseudo-random sets of sites, herein deletion or reserved sites, where nanoballs are prevented from attachment to the substrate so that the sites of the array can be used in a pattern matching scheme as registration markers for absolute location identification. Information can be extracted with a high degree of confidence that it is correlated to a known location, while at the same time the amount of information that can be packed on a chip is maximized.
First claim
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What is claimed is: 1. A method for use in performing biochemical experiments comprising: providing a microarray comprising a solid planar substrate having a surface; preparing a substrate for imaging to establish location and registration of biochemical sites on the substrate by providing a predetermined two-dimensional spatial pattern of biochemical sites in periodic distance between adjacent sites on the substrate; detecting image field spots in a spatial two-dimensional ar…
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- G06V10/24Primary
Physics · mapped topic
- C12Q1/6874Primary
Chemistry & Metallurgy · mapped topic
Physics · mapped topic
Physics · mapped topic
Physics · mapped topic
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- What does patent US9359641B2 cover?
- In a genome sequencing system and methodology, a protocol is provided to achieve precise alignment and accurate registration of an image of a planar array of nanoballs subject to optical analysis. Precise alignment correcting for fractional offsets is achieved by correcting for errors in subperiod x-y offset, scale and rotation by use of minimization techniques and Moiré averaging. In Moiré ave…
- Who is the assignee on this patent?
- Complete Genomics Inc
- What technology area does this patent fall under?
- Primary CPC classification G06V10/24. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Jun 07 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).