Valves and other flow control in fluidic systems including microfluidic systems

Patent metadata

Field	Value
Publication number	US-9358539-B2
Application number	US-99237609-A
Country	US
Kind code	B2
Filing date	May 15, 2009
Priority date	May 16, 2008
Publication date	Jun 7, 2016
Grant date	Jun 7, 2016

Abstract

Articles and methods for controlling flow in fluidic Systems, especially in microfluidic Systems, are provided. A microfluidic System includes a configuration such that the actuation of a single valve can allow the switching of fluids from a first fluid path (e.g., a first channel section) to a second fluid path (e.g., a second channel section). This may be achieved by incorporating a valve ( 38 ) with a first channel section ( 24 ), which may have a lower hydrodynamic resistance than a second channel section ( 28 ) prior to actuation of the valve. Actuation of the valve ( 38 ) can cause only the hydrodynamic resistance of the first channel section ( 24 ) to increase, thereby redirecting fluid flow into the second channel section ( 28 ) (which now has a relatively lower hydrodynamic resistance). The valve comprises a control channel ( 40 ) for introducing a positive or reduced pressure, and is adapted to modulate fluid flow in an adjacent channel section by constricting or expanding the channel section ( 24 ).

First claim

What is claimed is:

1. A microfluidic system, comprising:
a delivery channel;
a first channel section and a second channel section, wherein the first channel section and the second channel section are each downstream of and fluidly connected to the delivery channel at a junction, the second channel section having a higher hydrodynamic resistance than the first channel section;
a valve comprising a control channel adjacent the first channel section and not fluidly connected to the first channel section, wherein actuation of the valve adjusts hydrodynamic resistance of the first channel section, but does not result in variation in the amount of fluid flow through the second channel section; and
a membrane positioned between the first channel section and the control channel,
wherein the control channel is constructed and arranged to cause deflection of the membrane, resulting in constriction of at least a portion of the first channel section such that the first channel section has a higher hydrodynamic resistance than the second channel section,
wherein at least a portion of the control channel is on the same horizontal plane as the first channel section, and
wherein the second channel section does not include a valve able to vary its hydrodynamic resistance;
the microfluidic system further comprising a fluid containing droplets, the fluid contained within the delivery channel and flowing towards the junction into both the first channel section and the second channel section, wherein deflection of the membrane via the control channel controls the flow of droplets into the first channel section or the second channel section without preventing the flow of fluid into both the first channel section and the second channel section.

2. A microfluidic system as in claim 1 , comprising a substantially constant pressure source fluidly connected to the upstream portion of the delivery channel.

3. A microfluidic system as in claim 1 , comprising a substantially constant volume source fluidly connected to the upstream portion of the delivery channel.

4. A microfluidic system as in claim 1 , wherein the membrane has an average width of less than about 20 microns.

5. A microfluidic system as in claim 1 , wherein the membrane has an average width of about 10 microns to about 15 microns.

6. A microfluidic system as in claim 1 , wherein the length of the control channel is at least about 10 times the width of the first channel section.

7. A microfluidic system as in claim 1 , wherein the length of the control channel is at least about 500 microns.

8. A microfluidic system as in claim 1 , wherein the first channel section has an average aspect ratio of at least 2:1.

9. A microfluidic system as in claim 1 , wherein the Young's modulus of the membrane is from about 250 kPa to about 4000 kPa.

10. A microfluidic system as in claim 1 , wherein the valve is constructed and arranged to restrict fluid flow in the first channel section but not the second channel section.

11. A microfluidic system as in claim 1 , wherein substantially all of the control channel is on the same horizontal plane as the first channel section.

12. A microfluidic device as in claim 1 , wherein the valve is actuated by a pressure source fluidly connected to the control channel.

13. A method, comprising:
providing a microfluidic system comprising a delivery channel, a first channel section and a second channel section, each of the first channel section and the second channel section downstream of and fluidly connected to the delivery channel at a junction, the second channel section having a higher hydrodynamic resistance than the first channel section, wherein the microfluidic system further comprises a valve constructed and arranged to restrict fluid flow in the first channel section, the valve comprising a control channel adjacent the first channel section and not fluidly connected to the first channel section, and wherein the second channel section does not include a valve able to vary its hydrodynamic resistance;
flowing a fluid containing droplets through the delivery channel towards the junction, wherein the fluid flows into both the first channel section and the second channel section, and wherein droplets entering the junction flow into the first channel section but not the second channel section; and
actuating the valve to cause constriction of a portion of the first channel section to cause the first channel section to have a higher hydrodynamic resistance than the second channel section, wherein the droplets entering the junction flow into the second channel section but not into the first channel section while the fluid contained within the delivery channel flows into both the first channel section and the second channel section, and wherein actuation of the valve adjusts hydrodynamic resistance of the first channel section, but does not result in variation in the amount of fluid flow through the second channel section.

Assignees

• Abate Adam R
• Weitz David A
• Harvard College

Inventors

• Abate Adam R
• Weitz David A

Classifications

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• B01L2200/0636

  Focussing flows, e.g. to laminate flows · CPC title

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• B01L2200/146

  Employing pressure sensors · CPC title

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title