Sample plate for MALDI-TOF mass spectrometer and method of manufacturing the sample plate and mass spectrometry method using the sample plate

Patent metadata

Field	Value
Publication number	US-9355829-B2
Application number	US-201514800665-A
Country	US
Kind code	B2
Filing date	Jul 15, 2015
Priority date	Jul 16, 2014
Publication date	May 31, 2016
Grant date	May 31, 2016

Abstract

The present invention relates to a sample plate to be used for a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer, and more particularly, to a sample plate for a MALDI-TOF mass spectrometer, which is particularly useful for molecular weight measurement of a high-volatile material, a method of manufacturing the sample plate and a mass spectrometry method using the sample plate. Object of the present invention to provide a method capable of performing a mass spectrometry for a high-volatile material by using a MALDI-TOF mass spectrometer so as to overcome the limits of the gas chromatography method of the related art. According to the present invention, there is provided a sample plate including a target plate, an organic matrix formed on one surface of the target plate, a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix, and a sample fixing layer formed on the parylene thin film. The sample fixing layer is made of at least one material selected from a group consisting of graphene and carbon nano tube (CNT).

First claim

What is claim is:

1. A sample plate including:
a target plate,
an organic matrix formed on one surface of the target plate,
a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix, and
a sample fixing layer formed on the parylene thin film,
wherein the sample fixing layer is made of at least one material selected from a group consisting of graphene and carbon nano tube (CNT).

2. The sample plate of claim 1 , wherein the sample fixing layer is preferably made of graphene.

3. The sample plate of claim 1 , at least one material selected from the group consisting of graphene and carbon nano tube (CNT) may be mixed with a sample, which is an analysis target, and a mixture thereof may be formed on the parylene thin film, as the sample fixing layer.

4. The sample plate of claim 1 , the sample fixing layer may be formed on the parylene thin film without being mixed with a sample, which is an analysis target.

5. The sample plate of claim 4 , the sample plate may further include an analysis target formed on the parylene thin film.

6. The sample plate of claim 1 , the parylene thin film is formed to have a thickness of 10 nm to 100 nm.

7. The sample plate of claim 1 , the parylene thin film is a parylene-N thin film.

8. The sample plate of claim 1 , the sample plate is a sample plate for a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer.

9. A method of manufacturing a sample plate, the method including the steps of:
forming an organic matrix on one surface of a target plate,
forming a parylene thin film on the target plate having the organic matrix formed thereon so that it covers the entire organic matrix, and
forming a sample fixing layer by using at least one material selected from a group consisting of graphene and carbon nano tube (CNT).

10. The method of claim 9 , the step of forming the sample fixing layer may include steps of mixing at least one material selected from the group consisting of graphene and carbon nano tube with a sample, which is an analysis target, and forming a sample fixing layer mixed with the sample on the parylene thin film.

11. The method of claim 10 , the step of forming the sample fixing layer, the sample fixing layer may be formed on the parylene thin film without being mixed with a sample, which is an analysis target.

12. The method of claim 11 , the method may further include a step of forming the sample on the sample fixing layer.

13. The method of claim 10 , the parylene thin film is preferably parylene-N thin film.

14. The method of claim 10 , the step of forming the parylene thin film may include a first step of evaporating parylene dimer, a second step of thermally decomposing the evaporated parylene dimer to form an intermediate product, and a third step of introducing the intermediate product into a vapor deposition chamber and forming the parylene thin film on the target plate having the organic matrix formed thereon.

15. The method of claim 10 , the sample plate is preferably a sample plate for a MALDI-TOF mass spectrometer.

16. A mass spectrometry method using a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer, the method including the steps of:
providing a sample plate including a target plate, an organic matrix formed on one surface of the target plate, a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix, and a sample fixing layer formed on the parylene thin film, the sample fixing layer being made of at least one material selected from a group consisting of graphene and carbon nano tube (CNT);
forming a sample, which is an analysis target, on the sample fixing layer;
positioning the sample plate having the sample mounted thereon at a predetermined position of the MALDI-TOF mass spectrometer;
illuminating laser to the sample plate to desorb and ionize the sample, and
performing mass spectrometry for the ionized sample.

17. The method of claim 16 , the material mixed with the sample fixing layer or sample is preferably graphene.

18. A mass spectrometry method using a MALDI-TOF (Matrix Assisted Laser Desorption Ionization Time of Flight) mass spectrometer, the method including the steps of:
providing a sample plate including a target plate, an organic matrix formed on one surface of the target plate, and a parylene thin film formed on the target plate having the organic matrix formed thereon and formed to cover the entire organic matrix; mixing a sample, which is an analysis target, with at least one material selected from a group consisting of graphene and carbon nano tube (CNT);
forming the sample on the sample plate;
positioning the sample plate having the sample mounted thereon at a predetermined position of the MALDI-TOF mass spectrometer;
illuminating laser to the sample plate to desorb and ionize the sample, and
performing mass spectrometry for the ionized sample.

19. The method of claim 18 , the material mixed with a sample fixing layer or sample is preferably graphene.

Assignees

• Univ Yonsei Iacf

Inventors

• Pyun Jaechul
• Kim Jo Il

Classifications

• G01N1/28 Primary

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title

• H01J49/40

  Time-of-flight spectrometers (H01J49/36 takes precedence) · CPC title

• B05D1/60

  Deposition of organic layers from vapour phase (vapour phase deposition in general C23C14/00, C23C16/00) · CPC title

• H01J49/0418 Primary

  for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title