Pentose and glucose fermenting yeast cell

Patent metadata

Field	Value
Publication number	US-9353376-B2
Application number	US-201113822285-A
Country	US
Kind code	B2
Filing date	Oct 11, 2011
Priority date	Oct 13, 2010
Publication date	May 31, 2016
Grant date	May 31, 2016

Abstract

The present invention relates to a yeast cell comprising one or more exogenous genes of a pentose metabolic pathway non-native to the yeast cell wherein the yeast cell has a disruption of the hxk1, hxk2 glk1 and gal1 native in the yeast cell. The invention further relates to pentose and glucose fermenting yeast cell that is capable of simultaneous pentose and glucose consumption.

First claim

The invention claimed is:

1. A yeast cell comprising
at least one exogenous gene of at least one pentose metabolic pathway non-native to the yeast cell,
wherein said yeast cell has a disruption of the hexokinase 1 (hxk1), hexokinase 2 (hxk2), glucokinase 1 (glk1), and galactokinase 1 (gal1) native in the yeast cell, and
wherein said yeast cell further comprising at least one of re-introduced hxk1, hxk2, glk1, and gal1 gene, in order to restore an ability of said yeast to consume glucose.

2. A process for preparing a pentose fermenting yeast cell, comprising
subjecting a yeast cell comprising at least one exogenous gene of pentose metabolic pathway to disruption of the gene hxk2 native in the yeast cell,
subjecting a resulting disruptant strain to evolutionary engineering to improve pentose consumption, until the yeast cell has growth rate of at least 0.05 h −1 on the pentose as sole carbon source,
isolating the resulting pentose fermenting yeast cell, and
introducing in the resulting yeast, at least one of hxk1, hxk2, glk1, and gal1 gene, in order to restore an ability of said yeast to consume glucose.

3. A process, for preparing a pentose fermenting yeast cell, comprising
subjecting a yeast cell comprising at least one-exogenous gene of pentose metabolic pathway to disruption of the gene hxk2 native in the yeast cell, and
subjecting a resulting disruptant strain to evolutionary engineering to improve pentose consumption, until the yeast cell has growth rate of at least 0.05 h −1 on the pentose as sole carbon source, and
isolating the resulting pentose fermenting yeast cell,
wherein in the disruptant strain is the yeast cell of claim 1 .

4. A pentose and glucose fermenting Saccharomyces cell that is capable of anaerobic simultaneous pentose and glucose consumption, obtainable according to the process of claim 2 .

5. A process for producing a fermentation product from the fermentation of pentose, comprising:
culturing the pentose and glucose fermenting yeast cell according to claim 4 in a pentose and glucose containing material under suitable fermentation conditions for a period of time sufficient to allow the fermentation of pentose and glucose to fermentation product, wherein said yeast cell ferments pentose to produce fermentation product at a higher level relative to a corresponding wild-type yeast.

6. The process according to claim 5 , wherein the fermentation time is reduced relative to the corresponding fermentation of wild-type yeast.

7. The process according to claim 6 , wherein the fermentation time is reduced at least 40% or more.

8. The process according to claim 5 , wherein pentose and glucose are co-fermented.

9. The process according to claim 8 , wherein overall fermentation rate is at least about 20%, optionally at least about 50% or optionally about 100% higher than that of a process with a corresponding wild-type yeast.

10. The process according to claim 5 , wherein the pentose-containing material comprises a hydrolysate of lignocellulosic material.

11. The process according to claim 10 , wherein the hydrolysate is an enzymatic hydrolysate of lignocellulosic material.

Assignees

• Wisselink Hendrik Wouter
• Van Maris Antonius Jeroen Adriaan
• Pronk Jacobus Thomas
• Dsm Ip Assets Bv

Inventors

• Wisselink Hendrik Wouter
• Van Maris Antonius Jeroen Adriaan
• Pronk Jacobus Thomas

Classifications

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• C07K14/395 Primary

  from Saccharomyces · CPC title

• C12N15/81 Primary

  for yeasts · CPC title