Method for producing polymer particles, and polymer particles

Patent metadata

Field	Value
Publication number	US-9352298-B2
Application number	US-201113994495-A
Country	US
Kind code	B2
Filing date	Dec 14, 2011
Priority date	Dec 14, 2010
Publication date	May 31, 2016
Grant date	May 31, 2016

Abstract

Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A. a method for producing polysaccharide composite particles, the method including the following steps (1) to (3): (1) a step of preparing a polysaccharide solution, in which two or more kinds of polysaccharides are dissolved in an ionic liquid; (2) a step of preparing a droplet dispersion liquid of the polysaccharide solution, in which liquid droplets of the polysaccharide solution are dispersed in an organic solvent having low compatibility with the ionic liquid; and (3) a coagulation step in which a composite of the polysaccharides are coagulated to obtain the polysaccharide composite particles; and B. a method for producing a crosslinked polymer particle, the method including a step of allowing a polymer dissolved in an ionic liquid, to react with a crosslinking agent while the polymer is subjected to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

First claim

The invention claimed is:

1. A method for producing a polysaccharide composite particle, the method comprising:
dissolving two or more kinds of polysaccharides in an ionic liquid, thereby preparing a polysaccharide solution;
dispersing liquid droplets of the polysaccharide solution in an organic solvent having low compatibility with the ionic liquid, thereby preparing a droplet dispersion liquid of the polysaccharide solution; and
coagulating a composite of the polysaccharides, thereby obtaining the polysaccharide composite particle.

2. The method according to claim 1 , wherein the polysaccharides are two or more selected from the group consisting of cellulose, agarose, pullulan, and dextran.

3. The method according to claim 1 , wherein at least one of the polysaccharides is cellulose.

4. The method according to claim 1 , wherein the ionic liquid is an alkylimidazolium salt.

5. The method according to claim 1 , wherein the ionic liquid is 1-ethyl-3-methylimidazolium acetate, 1-butyl-3-methylimidazolium chloride, or a mixture thereof.

6. The method according to claim 1 , wherein the dispersing is carried out by mixing a solution comprising the organic solvent and an oleophilic polymer, with the polysaccharide solution.

7. A polysaccharide composite particle produced by the method according to claim 1 .

8. The polysaccharide composite particle according to claim 7 , wherein a linear flow rate at a column pressure of 0.4 MPa, when polysaccharide composite particles are packed in a column with an inner diameter of 0.5 cm to a height of 20.0 cm and water at 25° C. is allowed to flow through, is from 1600 cm/hour to 6000 cm/hour.

9. A filler; comprising:
the polysaccharide composite particle according to claim 7 ,
wherein the filler is suitable for chromatography.

10. An adsorbent comprising:
the polysaccharide composite particle according to claim 7 ,
wherein the adsorbent is suitable for purifying an antibody.

11. The adsorbent according to claim 10 , wherein the adsorbent comprises Protein A as an affinity ligand.

12. A method for producing a crosslinked polymer particle, the method comprising:
allowing a polymer dissolved in an ionic liquid, thereby reacting with a crosslinking agent while subjecting the polymer to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

13. The method according to claim 12 , further comprising:
adding a medium to a dispersion liquid,
wherein the medium is compatible with the ionic liquid and does not substantially dissolve the polymer to coagulate liquid droplets.

14. The method according to claim 12 , wherein the polymer is at least one member selected from the group consisting of a polysaccharide and a derivative thereof.

15. The method according to claim 14 , wherein the polysaccharide is at least one member selected from the group consisting of cellulose, agarose, chitosan, dextran, pullulan, and a derivative thereof.

16. The method according to claim 15 , wherein the polysaccharide comprises at least cellulose or a derivative thereof.

17. The method according to claim 12 , wherein the crosslinking agent is at least one member selected from the group consisting of a halohydrin, a bisepoxide, a polyepoxide, and divinylsulfone.

18. A crosslinked polymer particle produced by the method according to claim 12 .

19. The crosslinked polymer particle according to claim 18 , wherein a linear flow rate at a column pressure of 0.4 MPa, when crosslinked polymer particles are packed in a column with an inner diameter of 0.5 cm to a height of 20.0 cm and water at 25° C. is allowed to flow through, is from 1600 cm/hour to 6000 cm/hour.

20. A filler, comprising:
the crosslinked polymer particle according to claim 18 ,
wherein the filler is suitable for chromatography.

21. An adsorbent, comprising:
the crosslinked polymer particle according to claim 18 ,
wherein the adsorbent is suitable for purifying an antibody.

22. The adsorbent according to claim 21 , wherein the adsorbent comprises Protein A as an affinity ligand.

Assignees

• Nakamura Satoshi
• Fukuta Tetsuo
• Ikkyu Kazuhiro
• Otani Yu
• Kawai Hiroshi
• Jsr Corp

Inventors

• Nakamura Satoshi
• Fukuta Tetsuo
• Ikkyu Kazuhiro
• Otani Yu
• Kawai Hiroshi

Classifications

• B01J20/285 Primary

  based on polymers · CPC title

• C08L1/28

  Alkyl ethers · CPC title

• C08J3/24

  Crosslinking, e.g. vulcanising, of macromolecules (mechanical aspects B29C35/00; crosslinking agents C08K) · CPC title

• C08J3/16

  by coagulating dispersions {(C08J3/122 takes precedence)} · CPC title

• C08L1/02

  Cellulose; Modified cellulose · CPC title