Neurogenesis screening method and uses thereof

Patent metadata

Field	Value
Publication number	US-9351978-B2
Application number	US-201314044916-A
Country	US
Kind code	B2
Filing date	Oct 3, 2013
Priority date	Feb 29, 2012
Publication date	May 31, 2016
Grant date	May 31, 2016

Abstract

Provided herein are methods for detecting and/or confirming the neurogenesis effect of cholesterol and/or oxysterol utilizing adipose-derived stem cells (ADSCs). Further provided are methods of promoting neurogenesis in ADSCs. Also provided are methods for the use of cholesterol and/or oxysterol for the production of a neurologic component that may be incorporated into a nutritional composition for promoting neurogenesis in a pediatric subject.

First claim

We claim:

1. A method for the promoting neurogenesis in an infant, wherein neurogenesis is promoted in the infant administered the nutritional composition by a change in cell morphology selected from the group consisting of shrinkage of cytoplasm, formation of a neurite, formation of dendrite-like projection, and formation of an axon, as compared to an infant not administered the nutritional composition, wherein the method comprises the step of:
administering to the infant a nutritional composition comprising per 100 kcal:
from about 6 g to about 22 g carbohydrate source;
from about 1.3 g to about 7.2 g fat source;
from about 1.8 g to about 6.8 g a protein source; and
from about 5 mg about 100 mg of a neurologic component, wherein the neurologic component comprises at least 5 mg cholesterol and at least 5 mg of at least one oxysterol.

2. The method of claim 1 , wherein the nutritional composition comprises per 100 kcal from about 10 mg to 250 mg lactoferrin.

3. The method of claim 1 , wherein the nutritional composition comprises per 100 kcal from about 2.9 mg to about 17 mg β-glucan.

4. The method of claim 1 , wherein the nutritional composition comprises per 100 kcal from about 0.2 g to about 0.6 g polydextrose.

5. The method of claim 1 , wherein the nutritional composition comprises per 100 kcal 0.1 g to 1 g galacto-oligosaccharide.

6. The method of claim 1 , wherein the nutritional composition is an infant formula.

7. The method of claim 1 , wherein the nutritional composition comprises per 100 kcal from about 0.5 mg to about 45 mg sialic acid.

8. The method of claim 1 , wherein the protein source comprises hydrolyzed proteins having a degree of hydrolysis of between about 4% and 10%.

9. The method of claim 1 , wherein the protein source comprise from about 40% to about 85% whey protein and from about 15% to about 60% casein.

10. The method of claim 1 , wherein the protein source comprise extensively hydrolyzed protein.

11. The method of claim 1 , wherein the nutritional composition further comprises per 100 kcal from about 5 mg to about 100 mg arachidonic acid.

12. The method of claim 11 , wherein the nutritional composition comprises a weight ratio of arachidonic acid to docosahexaenoic acid of between about 1:2 to about 9:1.

13. The method of claim 1 , wherein the nutritional composition comprise per 100 kcal from about 9.60×10 5 to about 3.80×10 8 CFU of probiotics.

Assignees

• Mead Johnson Nutrition Co

Inventors

• Kuang Chenzhong
• Xiao Yan
• Hondmann Dirk
• Poels Eduard
• Jouni Zeina

Classifications

• G01N33/5058

  Neurological cells · CPC title

• A23L1/296

  Human Necessities · mapped topic

• G01N33/5073

  Stem cells · CPC title

• A61K31/575 Primary

  substituted in position 17 beta by a chain of three or more carbon atoms, e.g. cholane, cholestane, ergosterol, sitosterol · CPC title

• A23L33/40

  Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula · CPC title