Selectively targeted antimicrobial peptides and the use thereof

Patent metadata

Field	Value
Publication number	US-9351490-B2
Application number	US-201414178061-A
Country	US
Kind code	B2
Filing date	Feb 11, 2014
Priority date	Sep 6, 2006
Publication date	May 31, 2016
Grant date	May 31, 2016

Abstract

The present invention relates to targeting peptides capable of specifically binding to microbial organisms (e.g., P. aeruginosa or S. mutans ), antimicrobial peptides having antimicrobial activities, and specifically/selectively targeted antimicrobial peptides (STAMPs). In addition, the present invention provides methods of selectively killing or inhibiting microbial organisms by using the peptides or compositions provided by the present invention.

First claim

What is claimed is:

1. A method of selectively killing a Streptococcus mutans , said method comprising contacting said S. mutans with an amount of a construct effective to kill an S. mutans , wherein said construct comprises a targeting peptide that binds Streptococcus mutans , the amino acid sequence of said peptide consisting of the sequence TFFRLFNRSFTQALGK (SEQ ID NO:2) attached to a an antimicrobial peptide.

2. The method of claim 1 , wherein the amino acid sequence of said antimicrobial peptide comprises a novispirin amino acid sequence or a fragment thereof having antimicrobial activity.

3. The method of claim 1 , wherein the amino acid sequence of said antimicrobial peptide comprises the sequence KNLRIIRKGIHIIKKY (SEQ ID NO:3).

4. The method of claim 1 , wherein the amino acid sequence of said antimicrobial peptide consists of the sequence KNLRIIRKGIHIIKKY (SEQ ID NO:3).

5. The method according to claim 1 - 3 , or 4 , wherein the targeting peptide is attached to the antimicrobial peptide by a linker peptide.

6. The method of claim 5 , wherein the targeting peptide is attached to the antimicrobial peptide by a linker peptide, where the amino acid sequence of said linker peptide is selected from the group consisting of GGG (SEQ ID NO:17), AAA (SEQ ID NO:18), SAT (SEQ ID NO:19), ASA (SEQ ID NO:20), SGG (SEQ ID NO: 21), PYP (SEQ ID NO:22), SGS (SEQ ID NO:23), GGS (SEQ ID NO:24), SPS(SEQ ID NO:25), PSGSP (SEQ ID NO:26), PSPSP (SEQ ID NO:27), and GGSGGS (SEQ ID NO:28).

7. The method of claim 1 , wherein the carboxyl terminus of said antimicrobial peptide is amidated.

8. The method of claim 1 , wherein the targeting peptide attached to the antimicrobial peptide consists of the amino acid sequence TFFRLFNRSFTQALGKGGGKNLRIIRKGIHIIKKY (SEQ ID NO:4).

9. The method of claim 8 , wherein the carboxyl terminus of said peptide is amidated.

10. The method of claim 8 , wherein said S. mutans is on an oral soft tissue or on teeth.

11. The method of claim 8 , wherein said S. mutans is present in a biofilm.

12. The method of claim 1 , wherein said S. mutans is on an oral soft tissue or on teeth.

13. The method of claim 1 , wherein said S. mutans is present in a biofilm.

Assignees

• Univ California
• C3 Jian Inc

Inventors

• Eckert Randal H
• Yarbrough Daniel K
• Shi Wenyuan
• Anderson Maxwell H
• Qi Fengxia
• He Jian
• Mchardy Ian H

Classifications

• A61P31/04

  Antibacterial agents · CPC title

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title

• A61K47/65

  Peptidic linkers, binders or spacers, e.g. peptidic enzyme-labile linkers · CPC title

• A61K38/10 Primary

  Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)} · CPC title

• C07K2319/01

  containing a localisation/targetting motif · CPC title