Proteins used for the diagnosis of lyme borreliosis

Patent metadata

Field	Value
Publication number	US-9347943-B2
Application number	US-201013388178-A
Country	US
Kind code	B2
Filing date	Aug 26, 2010
Priority date	Aug 28, 2009
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

Chimera proteins including: (i) at least one sequence of a DbpA protein of a Borrelia species selected from B. afzelii, B. burgdorferi sensu stricto and B. garinii , and (ii) at least one sequence of an OspC protein of a Borrelia species selected from B. afzelii, B. burgdorferi sensu stricto and B. garinii . Also, a method and a kit for the in vitro diagnosis of Lyme borreliosis using said proteins.

First claim

The invention claimed is:

1. A polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequences of SEQ ID NOs: 8-14.

2. A diagnostic kit comprising the polypeptide as claimed in claim 1 .

3. The diagnostic kit as claimed in claim 2 , further comprising an anti-human-immunoglobulin labeled with a label.

4. The diagnostic kit as claimed in claim 2 , wherein the polypeptide is immobilized on a solid support.

5. The diagnostic kit as claimed in claim 2 , comprising (i) a first polypeptide comprising the amino acid sequence of SEQ ID NO: 9, (ii) a second polypeptide comprising the amino acid sequence of SEQ ID NO: 11, and (iii) a third polypeptide comprising the amino acid sequence of SEQ ID NO: 14.

6. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 8.

7. A diagnostic kit comprising the polypeptide as claimed in claim 6 and an anti-human-immunoglobulin labeled with a label.

8. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 9.

9. A diagnostic kit comprising the polypeptide as claimed in claim 8 and an anti-human-immunoglobulin labeled with a label.

10. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 10.

11. A diagnostic kit comprising the polypeptide as claimed in claim 10 and an anti-human-immunoglobulin labeled with a label.

12. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 11.

13. A diagnostic kit comprising the polypeptide as claimed in claim 12 and an anti-human-immunoglobulin labeled with a label.

14. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 12.

15. A diagnostic kit comprising the polypeptide as claimed in claim 14 and an anti-human-immunoglobulin labeled with a label.

16. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 13.

17. A diagnostic kit comprising the polypeptide as claimed in claim 16 and an anti-human-immunoglobulin labeled with a label.

18. The polypeptide as claimed in claim 1 , comprising the amino acid sequence of SEQ ID NO: 14.

19. A diagnostic kit comprising the polypeptide as claimed in claim 18 and an anti-human-immunoglobulin labeled with a label.

20. A method for the in vitro diagnosis of Lyme borreliosis using a biological sample, comprising:
bringing the biological sample into contact with at least one polypeptide as claimed in claim 1 ; and
determining whether there is formation of an immunological complex between the polypeptide and antibodies of the biological sample.

21. The method as claimed in claim 20 , wherein the antibodies of the biological sample are IgGs and/or IgMs.

22. The method as claimed in claim 21 , wherein the formation of the immunological complex is determined by adding at least one anti-human-immunoglobulin labeled with a label.

23. The method as claimed in claim 20 , wherein the polypeptide is immobilized on a solid support.

24. The method as claimed in claim 21 , wherein the polypeptide is immobilized on a solid support.

25. The method as claimed in claim 22 , wherein the polypeptide is immobilized on a solid support.

Assignees

• Levet Lionel
• Mejan-Letourneur Odile
• Biomerieux Sa

Inventors

• Levet Lionel
• Mejan-Letourneur Odile

Classifications

• A61P37/04

  Immunostimulants · CPC title

• C07K14/195

  from bacteria · CPC title

• G01N2333/20

  from Spirochaetales (O), e.g. Treponema, Leptospira · CPC title

• G01N2469/20

  Detection of antibodies in sample from host which are directed against antigens from microorganisms · CPC title

• C07K14/20

  from Spirochaetales (O), e.g. Treponema, Leptospira · CPC title