Genetic analyzer

Patent metadata

Field	Value
Publication number	US-9347096-B2
Application number	US-201213982544-A
Country	US
Kind code	B2
Filing date	Jan 23, 2012
Priority date	Jan 31, 2011
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

A nucleic acid analysis apparatus capable of selecting an optimal analysis method for every user and improving throughput is provided. In a genetic analyzer for measuring and analyzing amplification reaction of a nucleic acid in real time, an amplification curve is analyzed and a user can select conditions for terminating the amplification reaction upon detection of amplification. Further, a user can select conditions for selecting next processing after termination of the amplification reaction. A user can select, in situ, conditions for terminating the amplification reaction and conditions for selecting next processing upon detection of amplification and after the termination of amplification reaction. Alternatively, conditions for terminating the amplification reaction and conditions for selecting the next processing are registered previously and processing is performed automatically upon detection of amplification and after termination of the amplification reaction.

First claim

The invention claimed is:

1. A genetic analyzer for measuring and analyzing an amplification reaction of a nucleic acid in real time, comprising:
a nucleic acid amplification device for conducting nucleic acid amplification and detecting fluorescence in the course of amplification;
a control unit coupled to the nucleic acid amplification device;
an input section coupled to the control unit;
a display coupled to the control unit,
wherein the control unit is programmed to display on the display a screen enabling selection, by the input section, of one of a plurality of analysis conditions of the nucleic acid amplification, which include:
terminating an amplification reaction in which the amplification reaction is to be terminated instantly,
continuing the amplification reaction until elapse of a measurement completion time and then terminating the amplification reaction, and
continuing the amplification reaction until elapse of a predetermined time and then terminating the amplification reaction,
wherein the nucleic acid amplification device includes a plurality of holding units disposed around a circumference of a disk that is rotatable and each holding unit has a temperature control block holding at least one reaction vessel containing a nucleic acid sample as a target for amplification processing and a reagent as a reaction solution,
wherein each of the temperature control blocks is configured to control the temperature of the reaction solution and wherein the temperature control blocks are disposed apart from each other, and
wherein the control unit is programmed to receive an input indicating a selection selected through the input section of one of the analysis conditions displayed on the screen for each nucleic acid sample contained in a reaction vessel, and the displayed analysis conditions are conditions for each nucleic acid sample for terminating the amplification reaction,
wherein the control unit is further programmed to:
control the nucleic acid amplification device to terminate the amplification reaction of a reaction solution according to the selected analysis condition corresponding to the sample in the reaction solution, and
receive one or more inputs indicating selections selected through the input section of one of the analysis conditions displayed on the display screen for each of the nucleic acid samples before nucleic acid amplification of a nucleic acid sample is conducted by the nucleic acid amplification device.

2. The genetic analyzer according to claim 1 , wherein said control unit is further programmed to process detection of an occurrence of the amplification reaction from a cycle threshold (Ct) value of the intensity of detected fluorescence.

3. The genetic analyzer according to claim 1 , wherein said control unit is further programmed to process a detection of an occurrence of the amplification reaction from a plateau of the intensity of detected fluorescence.

4. The genetic analyzer according to claim 1 , wherein said control unit is further programmed to process a detection of an occurrence of the amplification reaction from a cycle threshold (Ct) value or a plateau of the intensity of detected fluorescence, which a user can previously select.

5. The genetic analyzer according to claim 1 , wherein said control unit is further programmed to process a detection of an occurrence of the amplification reaction from intensity of the detected fluorescence, and wherein the displayed analysis conditions to be selected by the user include conditions for detecting occurrence of the amplification reaction.

6. The genetic analyzer according to claim 1 , wherein the control unit is further programmed to display an amplification reaction curve on the display.

7. The genetic analyzer according to claim 1 , wherein the holding unit has a mechanism of successively carrying in the reaction vessels.

8. The genetic analyzer according to claim 1 , wherein the holding unit has a mechanism of successively carrying out the reaction vessels.

9. The genetic analyzer according to claim 1 , wherein the nucleic acid amplification device continuously performs the amplification reaction of the nucleic acid, processing of a Melting analysis or HRM analysis, or a thermal treatment of deactivating an enzyme without moving the reaction vessel.

10. The genetic analyzer according to claim 1 , wherein the plurality of temperature control blocks are configured to control the temperature of the reaction solution independently from each other.

11. A genetic analyzer for measuring and analyzing an amplification reaction of a nucleic acid in real time, comprising:
a nucleic acid amplification device for conducting nucleic acid amplification and detecting fluorescence in the course of amplification;
a control unit coupled to the nucleic acid amplification device,
wherein said control unit is programmed to process a detection of an occurrence of the amplification reaction from an intensity of the detected fluorescence,
wherein the nucleic acid amplification device includes a plurality of holding units disposed around a circumference of a disk that is rotatable and each holding unit has a temperature control block holding one reaction vessel containing a nucleic acid sample as a target for amplification processing and a reagent as a reaction solution,
wherein each of the temperature control blocks is configured to control the temperature of the reaction solution and are disposed apart from each other, and
wherein the control unit is programmed to: when the occurrence of the amplification reaction has been detected before a predetermined measurement time has elapsed, processes predetermined user input that is a selection of analysis conditions which include conditions for terminating the amplification reaction, including:
terminating the amplification reaction in which the amplification reaction is to be terminated instantly,
continuing the amplification reaction until elapse of the measurement completion time and then terminating the amplification reaction, and
continuing the amplification reaction until elapse of a predetermined time and then terminating the amplification reaction, and
wherein said control unit is further programmed to:
control the nucleic acid amplification device to terminate the amplification reaction automatically in accordance with the predetermined condition for terminating the amplification reaction selected by the user, and
process one or more inputs indicating selections of the analysis conditions for each of the nucleic acid samples before nucleic acid amplification of a nucleic acid sample is conducted by the nucleic acid amplification device.

12. The genetic analyzer according to claim 11 , further comprising reaction vessels for containing a nucleic acid sample as a target for amplification processing and a reagent, wherein the conditions for terminating the amplification reaction can be set for every reaction vessel.

13. The genetic analyzer according to claim 11 , further comprising a display,
wherein the control unit is further programmed to display conditions for selecting a next processing and, when the amplification reaction is terminated, the control unit receives a section of a condition for selecting next processing through the input section.

14. The genetic analyzer according to claim 13 , wherein said control unit is further programmed to process predetermined user input that sets the conditions for the next processing and, when the amplification reaction is terminated, the next processing is performed automatically in accordance with the predetermined conditions set for the next proc

Assignees

• Morishima Daisuke
• Maeda Kohshi
• Shimane Nobuyoshi
• Hitachi High Tech Corp

Inventors

• Morishima Daisuke
• Maeda Kohshi
• Shimane Nobuyoshi

Classifications

• B01L7/52

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• C12Q2527/107

  Temperature of melting, i.e. Tm · CPC title

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title

• C12Q1/6846

  Common amplification features · CPC title

• G01N35/0092 Primary

  Scheduling · CPC title