Use of browned glucose as a feed substrate

Patent metadata

Field	Value
Publication number	US-9347080-B2
Application number	US-201213982678-A
Country	US
Kind code	B2
Filing date	Jan 25, 2012
Priority date	Jan 31, 2011
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

A method for fermenting a microorganism, producing a compound of interest, in a culture medium comprising: adding a browned glucose solution to the culture medium, wherein the browned glucose solution is a glucose solution that has been acid treated and heated to a temperature of at least 90 degrees Celsius, and wherein the glucose solution has a concentration of at least 500 g/l.

First claim

The invention claimed is:

1. A method for fermenting a microorganism, producing a compound of interest, in a culture medium comprising the steps of:
a) inoculating a culture medium in a fermenter with a microorganism capable of producing a compound of interest;
b) adding a browned glucose solution to the culture medium, wherein the browned glucose solution is a glucose solution that has been acid treated and heated to a temperature of at least 90 degrees Celsius, and wherein the glucose solution has a concentration of at least 500 g/l;
c) fermenting the microorganism to provide a fermentation broth; and
d) recovering the compound of interest from the fermentation broth.

2. The method according to claim 1 , wherein the microorganism is a bacterium or a fungus.

3. The method according to claim 2 , wherein the bacterium is selected from the group consisting of Bacillus, Streptomyces, Escherichia , and Pseudomonas.

4. The method according to claim 2 , wherein the fungus is selected from the group consisting of Achlya, Aspergillus, Cephalosporium, Cochliobolus, Endothia, Fusarium, Humicola, Mucor, Neurospora, Penicillium, Podospora, Pyricularia , and Trichoderma.

5. The method according to claim 1 , wherein the compound of interest is a secondary metabolite.

6. The method according to claim 1 , wherein the compound of interest is a protein.

7. The method according to claim 6 , wherein the protein is an enzyme.

8. The method according to claim 1 , wherein the fermentation is a batch, a fed batch, a repeated fed batch or a continuous fermentation.

9. The method according to claim 1 , wherein the acid treatment results in a glucose solution with a pH less than pH 4.5.

10. The method according to claim 1 , wherein the glucose solution is acid treated and thereafter heated to a temperature of at least 90 degrees Celsius.

11. The method according to claim 1 , wherein the glucose solution has a concentration of from 500 g/l to 1200 g/l.

12. The method according to claim 1 , wherein the compound of interest is recovered after removal of the microorganism.

13. The method according to claim 5 , wherein the secondary metabolite is Thaxtomin.

14. A method for fermenting a microorganism and producing a compound of interest in a culture medium comprising the steps of:
a) inoculating a culture medium in a fermenter with a microorganism capable of producing a compound of interest;
b) adding a browned glucose solution to the culture medium, wherein the browned glucose solution is a glucose solution that has been acid treated and heated to a temperature of at least 90 degrees Celsius, and wherein the glucose solution has a concentration of at least 500 g/l; and
c) fermenting the microorganism to provide a fermentation broth.

15. The method according to claim 14 , wherein the acid treated glucose solution has a pH less than pH 4.5.

16. The method according to claim 14 , wherein the glucose solution has a concentration of from 500 g/l to 1200 g/l.

Assignees

• Banke Niels
• Novozymes As

Inventors

• Banke Niels

Classifications

• C12N1/38 Primary

  Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) · CPC title

• C12P17/165

  Heterorings having nitrogen atoms as the only ring heteroatoms · CPC title

• C12P21/02 Primary

  having a known sequence of two or more amino acids, e.g. glutathione · CPC title