Production of dihydrosterculic acid and derivatives thereof

Patent metadata

Field	Value
Publication number	US-9347067-B2
Application number	US-201214369540-A
Country	US
Kind code	B2
Filing date	Dec 21, 2012
Priority date	Dec 27, 2011
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

The present invention relates to recombinant cells, particularly recombinant plant cells, which are capable of producing dihydrosterculic acid and/or derivatives thereof. The present invention also relates to methods of producing oil comprising dihydrosterculic acid and/or derivatives thereof.

First claim

The invention claimed is:

1. A process for producing extracted plant oil containing dihydrosterculic acid (DHS) and/or a fatty acid derivative thereof, the process comprising
i) obtaining recombinant plant cells comprising DHS, and/or a fatty acid derivative thereof, the DI-IS or fatty acid derivative thereof being esterified in triacylglycerols in the cells, wherein at least 7% of the total fatty acid in extractable oil in the cells is DHS and/or a fatty acid derivative thereof, wherein the recombinant plant cells are comprised in a leaf, and,
ii) extracting oil from the cells so as to thereby produce the extracted plant oil.

2. The process of claim 1 , wherein step i) comprises obtaining pieces of leaf tissue from at least 100 plants, which on average comprise at least about 7% DHS as a percentage of the total fatty acids in the extractable oil in the leaf tissues.

3. The process of claim 1 , wherein at least 10% of the total fatty acid in the extracted oil is DHS and/or a fatty acid derivative thereof.

4. The process of claim 1 , wherein
i) the fatty acid derivative comprises a cyclo-propyi group or is a branched chain fatty acid having a methyl group as the branch and/or
ii) the derivative comprises 2, 4 or 6 more carbons in the acyl chain when compared to DHS.

5. The process of claim 4 , wherein the fatty acid derivative comprising a cyclo-propyl group or a methyl group is isosteanic acid. with the methyl croup attached to C9 or C10.

6. The process of claim 1 , wherein there is no detectable fatty acid. derivative of DHS present in the extracted oil.

7. The process of claim 1 , wherein the recombinant plant cells comprise an exogenous polynucleotide which encodes a cyclopropane fatty acid synthetase (CPFAS).

8. The process of claim 1 , wherein at least 7% of the total fatty acid content in the extracted oil is DHS.

9. The process of claim 1 , wherein at least 7% of the total fatty acid content in the extracted oil is isostearic acid with a methyl group attached to C9 or C10.

10. the process of claim 1 , wherein the extracted oil has a ratio of oleic acid to DHS and/or fatty acid derivative thereof in its total fatty acid content of less than 2:1.

11. The process of claim 10 , wherein the ratio of oleic acid to DHS and/or fatty acid, derivative thereof is less than 1.5:1.

12. The process of claim 1 , wherein less than 10% sterculic acid and/or malvalic acid is present in the extracted oil.

Assignees

• Wood Craig Christopher
• Naim Fatima
• Singh Surinder Pal
• Okada Shoko
• Commw Scient Ind Res Org

Inventors

• Wood Craig Christopher
• Naim Fatima
• Singh Surinder Pal
• Okada Shoko

Classifications

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C11B3/001

  by a combination of two or more of the means hereafter · CPC title

• C12P7/6409

  Fatty acids · CPC title

• C12N15/80

  for fungi · CPC title

• C12P7/6436

  Fatty acid esters · CPC title