Container for forming a cell aggregate and a method for forming a cell aggregate

Patent metadata

Field	Value
Publication number	US-9347031-B2
Application number	US-201013377850-A
Country	US
Kind code	B2
Filing date	Jun 15, 2010
Priority date	Jun 15, 2009
Publication date	May 24, 2016
Grant date	May 24, 2016

Abstract

A container for forming a cell aggregate is provided in which a group represented by a general formula of:

(in the formula, each of R 1 , R 2 , and R 3 is independently a an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less.) and at least one of an amino group, a carboxyl group, and a hydroxyl group are present near a surface thereof.

First claim

The invention claimed is:

1. A container for forming a cell aggregate,
wherein the container is manufactured by reacting
a compound including
a functional group, and
a group represented by a general formula of:

wherein each of R 1 , R 2 , and R 3 is independently an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less, wherein a molecular weight of the compound is 225 or more and 650 or less, with

an unreacted container with a carboxyl group, an amino group, and a hydroxyl group on a surface thereof;

a) wherein the functional group of the compound has a reactivity with a carboxyl group and is an amino group or a hydroxyl group so that a condensation reaction between an amino group or a hydroxyl group of the compound and a carboxyl group on the surface of the unreacted container occurs, and at least an amino group and a hydroxyl group of the unreacted container are left on the surface of the container, or
b) wherein the functional group of the compound has a reactivity with an amino group or a hydroxyl group and is a carboxyl group or an aldehyde group so that a condensation reaction between a carboxyl group or an aldehyde group of the compound and an amino group or a hydroxyl group on the surface of the unreacted container occurs, and, for part b), at least a carboxyl group of the unreacted container is left on the surface of the container.

2. The container for forming a cell aggregate as claimed in claim 1 , wherein the unreacted container is plasma-treated with under an atmosphere including at least one of oxygen, ozone, and a water vapor, and ammonia and/or nitrogen.

3. A container for forming a cell aggregate,
wherein the container is manufactured by reacting
a first compound having
a first alkoxysilyl group that is a functional group capable of producing a silanol group through hydrolysis thereof, and
a group represented by a general formula of:

wherein each of R 1 , R 2 , and R 3 is independently an alkyl group with a carbon number of 1 or more and 6 or less and m is an integer of 2 or more and 6 or less, wherein a molecular weight of the first compound is 315 or more and 650 or less, and

a second compound having a second alkoxysilyl group that is a functional group capable of producing a silanol group through hydrolysis thereof and an amino group, with
an unreacted container with silanol groups on a surface thereof,
to cause at least
a condensation reaction between a silanol group produced by hydrolysis of the first alkoxysilyl group of the first compound and a silanol group on the surface of the unreacted container and
a condensation reaction between a silanol group produced by hydrolysis of the second alkoxysilyl group of the second compound and a silanol group on the surface of the unreacted container,

so that the group represented by a general formula of the first compound and an amino group of the second compound is present on the surface of the container.

Assignees

• Fujiwara Shigeyoshi
• Takei Keigo
• Hirayama Aya
• Suda Yukimitsu
• Shiseido Co Ltd

Inventors

• Fujiwara Shigeyoshi
• Takei Keigo
• Hirayama Aya
• Suda Yukimitsu

Classifications

• C12N5/0693

  Tumour cells; Cancer cells · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12N5/062

  Sensory transducers, e.g. photoreceptors; Sensory neurons, e.g. for hearing, taste, smell, pH, touch, temperature, pain · CPC title

• C12N5/0671

  Three-dimensional culture, tissue culture or organ culture; Encapsulated cells · CPC title

• C12N5/0667

  Adipose-derived stem cells [ADSC]; Adipose stromal stem cells · CPC title