Devices and methods for adaptive micro-gas chromatography

Patent metadata

Field	Value
Publication number	US-9341604-B2
Application number	US-201214357157-A
Country	US
Kind code	B2
Filing date	Nov 8, 2012
Priority date	Nov 9, 2011
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

The present disclosure provides adaptive methods for gas chromatography analysis of a gas sample comprising one or more target analytes (such as a micro-gas chromatography) and adaptive gas chromatography devices for carrying out such analytical methods. Broadly, the system can regulate flow into a downstream chromatographic column by detecting one or more upstream conditions. For example, one adaptive chromatography device comprises a first column, a modulator component, and a second column. A first detector or sensor detects the presence of target analytes upstream from the second column, while a second detector detects the presence of target analytes eluted from the second column. The modulator component assembly is responsive to an output generated by the first detector and adaptively regulates fluid flow into the second column. Such adaptive chromatography (micro-GC) systems have higher separation speed, better analyte identification capability, and far greater energy savings.

First claim

What is claimed is:

1. An adaptive chromatography device, comprising:
a first chromatographic column that receives a sample comprising one or more target analytes;
a modulator component assembly disposed downstream of and in fluid communication with the first chromatographic column, wherein the modulator component assembly comprises:
a first detector for detecting the presence of one or more target analytes eluted from the first chromatographic column and a thermal injector device; and
a valve, wherein in a first position, the valve permits fluid to flow from the first chromatographic column into the second chromatographic column and in a second position, the valve prevents fluid from flowing from the first chromatographic column into the second chromatographic column, wherein the output generated by the first detector is capable of changing the valve from the first position to the second position;

a second chromatographic column disposed downstream of and in fluid communication with the modulator component assembly; and
a second detector for detecting the presence of one or more target analytes eluted from the second chromatographic column; wherein the modulator component assembly is responsive to an output generated by the first detector to regulate fluid flow into the second chromatographic column.

2. The adaptive chromatography device of claim 1 , wherein the valve is a three-way valve and in the second position the valve receives a supplemental carrier fluid that flows with the sample into the second chromatographic column.

3. The adaptive chromatography device of claim 1 , further comprising at least one additional chromatographic column disposed downstream from and in fluid communication with the modulator component assembly and the first chromatographic column, wherein the modulator component assembly also regulates fluid flow from the first chromatographic column into the at least one additional chromatographic column based on the output generated by the first detector.

4. The adaptive chromatography device of claim 1 , wherein the first detector is a non-destructive on-column detector selected from the group consisting of: a capillary based optical ring resonator (CBORR) device, a Fabry-Perot interferometer based sensor, a chemi-resistor sensor, a sound acoustic wave sensor, and a thermal conductivity sensor.

5. The adaptive chromatography device of claim 1 , wherein the output generated by the first detector is a first output and a second output is generated by the second detector, wherein the modulator component assembly is responsive to both the first output and the second output to regulate fluid flow in both the first chromatographic column and the second chromatographic column.

6. The adaptive chromatography device of claim 1 , wherein the first chromatographic column is a first micro-gas chromatographic column and the second chromatographic column is a second micro-gas chromatographic column.

7. The adaptive chromatography device of claim 6 , further comprising at least one additional micro-gas chromatographic column downstream from and in fluid communication with the modulator component assembly, wherein the modulator component assembly also regulates fluid flow into the at least one additional micro-gas chromatographic column.

8. The adaptive chromatography device of claim 7 , wherein the at least one additional micro-gas chromatographic column is configured in series with the second micro-gas chromatographic column downstream from and in fluid communication with the modulator component assembly, and the modulator component assembly selectively controls fluid flow into the second micro-gas chromatographic column or the at least one additional micro-gas chromatographic column based on the output generated by the first detector.

9. An adaptive micro-gas chromatography device, comprising:
a first micro-gas chromatographic column, wherein the first micro-gas chromatographic column receives a sample comprising one or more target analytes; and
a modulator component assembly disposed downstream of and in fluid communication with the first micro-gas chromatographic column, which regulates fluid flow into a plurality of downstream micro-gas chromatographic columns in fluid communication therewith, wherein the modulator component assembly comprises:
a first on-column detector for detecting the presence of one or more target analytes eluted from the first micro-gas chromatographic column and a thermal injector device, wherein the adaptive micro-gas chromatography device further comprises an additional detector for each respective downstream micro-gas chromatographic column so as to detect the presence of one or more target analytes eluted therefrom; wherein the first on-column detector generates an output received by the modulator component assembly to regulate fluid flow into the respective downstream micro-gas chromatographic columns; and
a flow regulating device disposed between the first micro-gas chromatographic column and the plurality of downstream micro-gas chromatographic columns, wherein in a first position, the flow regulating device permits fluid to flow from the first micro-gas chromatographic column into at least one of the plurality of downstream micro-gas chromatographic columns and in a second position, the flow regulating device prevents fluid from flowing from the first micro-gas chromatographic column into at least one of the plurality of downstream micro-gas chromatographic columns, wherein the output from the first on-column detector is capable of changing the flow regulating device from the first position to the second position.

10. The adaptive micro-gas chromatography device of claim 9 , wherein the first on-column detector is a non-destructive detector selected from the group consisting of: a capillary based optical ring resonator (CBORR) device, a Fabry-Perot interferometer based sensor, a chemi-resistor sensor, a sound acoustic wave sensor, and a thermal conductivity sensor.

11. The adaptive micro-gas chromatography device of claim 10 , wherein the output generated by the first on-column detector is a first output and at least one additional output is generated by at least one of the additional detectors of the plurality of downstream micro-gas chromatographic columns, wherein the modulator component assembly is responsive to both the first output and the at least one additional output to regulate fluid flow in both the first micro-gas chromatographic column and the plurality of downstream micro-gas chromatographic columns.

12. A method of adaptive chromatography analysis comprising:
separating a sample in a first chromatographic column;
generating an output signal by detecting one or more target analytes during or after the separating of the sample in the first chromatographic column;
adaptively regulating flow into at least one downstream chromatographic column based on the generated output signal to further separate the sample in the at least one downstream chromatographic column so as to analyze at least a portion of the one or more target analytes; and
generating a baseline output signal in the absence of the one or more target analytes in the first chromatographic column and further generating a peak output signal in the presence of the one or more target analytes in the first chromatographic column, so that after the generated output signal reaches the peak output signal and falls to the baseline output signal, flow between the first chromatographic column into the at least one downstream chromatographic column is inhibited for a predetermined period to permit the sample to pass through the at least one downstream chromatographic column.

Assignees

• Univ Michigan

Inventors

• Fan Xudong
• Gianchandani Yogesh B
• Liu Jing

Classifications

• G01N30/465

  with specially adapted interfaces between the columns · CPC title

• G01N30/463 Primary

  for multidimensional chromatography · CPC title

• G01N30/466

  with separation columns in parallel · CPC title

• G01N30/46

  using more than one column {(G01N30/44 takes precedence)} · CPC title

• G01N30/72

  Mass spectrometers {(mass spectrometers per se H01J49/00)} · CPC title