Accumulation of omega-7 fatty acids in plant seeds

Patent metadata

Field	Value
Publication number	US-9340776-B2
Application number	US-201113168320-A
Country	US
Kind code	B2
Filing date	Jun 24, 2011
Priority date	Jun 24, 2010
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

Compositions and methods include genetically encoding and expressing a novel Δ 9 -18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel Δ 9 -18:0-ACP desaturase. In other embodiments, amino acid sequences have Δ 9 -18:0-ACP desaturase activity. Methods can involve expression of Δ 9 -18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

First claim

What is claimed is:

1. A method for producing a transgenic plant material, the method comprising:
introducing a nucleic acid molecule into a plant material comprising an LnΔ9D or AnΔ9 desaturase,
wherein the nucleic acid molecule comprises a polynucleotide that is at least 60% identical to SEQ ID NO:1 and encodes a plastidial delta-9 desaturase having at least 90% identity to SEQ ID NO:2.

2. The method of claim 1 , wherein introducing the nucleic acid molecule comprises transforming the plant material with the nucleic acid molecule.

3. The method of claim 1 , wherein the plant material comprises a means for increasing levels of 16:0-ACP in the plant material.

4. The method of claim 3 , wherein the means for increasing levels of 16:0-ACP in the plant material is suppression of KASII.

5. The method of claim 4 , wherein suppression of KASII is accomplished by introducing a mutation in the fab1 gene.

6. The method of claim 3 , wherein the means for increasing levels of 16:0-ACP in the plant material is decreasing the elongation of 16:0 fatty acids in the plant material.

7. The method of claim 6 , wherein decreasing the elongation of 16:0 fatty acids in the plant material is accomplished by introducing a mutation in the fae1 gene.

8. The method of claim 1 , wherein the plant material is obtained from a plant selected from a genus selected from the group comprising Arabidopsis, Borago, Canola, Ricinus, Theobroma, Zea ), Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes, Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis, rapeseed, Carthamus, Glycine, Soja, Helianthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale , or other members of the Gramineae.

9. The method of claim 1 , wherein the plant material comprises two means for increasing levels of 16:0-ACP in the plant material.

10. The method of claim 9 , wherein the first means for increasing levels of 16:0-ACP in the plant material is suppression of KASII, and wherein the second means for increasing levels of 16:0-ACP in the plant material is decreasing the elongation of 16:0 fatty acids in the plant material.

11. The method of claim 1 , wherein the polynucleotide encodes a polypeptide comprising a serine at the position analogous to position 114 in SEQ ID NO:2; an arginine at the position analogous to position 117 in SEQ ID NO:2; a cysteine at the position analogous to position 118 in SEQ ID NO:2, a leucine at the position analogous to position 179 in SEQ ID NO:2; or a threonine at the position analogous to position 188 in SEQ ID NO:2.

12. The method of claim 1 , wherein the polynucleotide encodes a polypeptide comprising a serine at the position analogous to position 114 in SEQ ID NO:2; an arginine at the position analogous to position 117 in SEQ ID NO:2; a cysteine at the position analogous to position 118 in SEQ ID NO:2, a leucine at the position analogous to position 179 in SEQ ID NO:2; and a threonine at the position analogous to position 188 in SEQ ID NO:2.

13. The method of claim 1 , wherein the polynucleotide encodes a polypeptide having at least 95% identity to SEQ ID NO:2.

14. The method of claim 1 , wherein the polynucleotide encodes a polypeptide having at least 98% identity to SEQ ID NO:2.

15. The method of claim 1 , wherein the polynucleotide encodes the polypeptide of SEQ ID NO:2.

16. The method of claim 1 , wherein the plant material is a plant.

17. A transgenic plant material comprising:
a polynucleotide at least 60% identical to SEQ ID NO:1, wherein the polynucleotide encodes a plastidial delta-9 desaturase at least 90% identical to SEQ ID NO:2; and an LnΔ9D or AnΔ9 desaturase.

18. The transgenic plant material of claim 17 , wherein the plant material comprises a means for increasing levels of 16:0-ACP in the plant material.

19. The transgenic plant material of claim 18 , wherein the means for increasing levels of 16:0-ACP in the plant material is suppression of KASII.

20. The transgenic plant material of claim 18 , wherein the means for increasing levels of 16:0-ACP in the plant material is decreasing the elongation of 16:0 fatty acids in the plant material.

21. The transgenic plant material of claim 17 , wherein the plant material is obtained from a plant selected from a genus selected from the group comprising Arabidopsis, Borago, Canola, Ricinus, Theobroma, Zea ), Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes, Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis , rapeseed, Carthamus, Glycine, Soja, Helianthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale , or other members of the Gramineae.

22. The transgenic plant material of claim 17 , wherein the plant material comprises a mutant fab1 gene or a mutant fae1 gene.

23. The transgenic plant material of claim 17 , wherein the plant material comprises a mutant fab1 gene and a mutant fae1 gene.

24. The transgenic plant material of claim 17 , wherein the plant material is a plant or a seed.

25. A method for producing a transgenic plant material, the method comprising introducing into a plant material:
a nucleic acid molecule comprising a polynucleotide that is at least 60% identical to SEQ ID NO:1 and encodes a plastidial delta-9 desaturase having at least 90% identity to SEQ ID NO:2; and
a nucleic acid molecule encoding an LnΔ9D or AnΔ9 desaturase.

26. The method of claim 25 , wherein the plant material comprises a means for increasing levels of 16:0-ACP in the plant material.

27. The method of claim 26 , wherein the means for increasing levels of 16:0-ACP in the plant material is suppression of KASII.

28. The method of claim 26 , wherein the means for increasing levels of 16:0-ACP in the plant material is decreasing the elongation of 16:0 fatty acids in the plant material.

29. The method of claim 25 , wherein the plant material is obtained from a plant selected from a genus selected from the group comprising Arabidopsis, Borago, Canola, Ricinus, Theobroma, Zea ), Gossypium, Crambe, Cuphea, Linum, Lesquerella, Limnanthes, Linola, Tropaeolum, Oenothera, Olea, Elaeis, Arachis, rapeseed, Carthamus, Glycine, Soja, Helianthus, Nicotiana, Vernonia, Triticum, Hordeum, Oryza, Avena, Sorghum, Secale , or other members of the Gramineae.

30. The method of claim 25 , wherein the plant material comprises a mutant fab1 gene or a mutant fae1 gene.

31. The method of claim 25 , wherein the plant material comprises a mutant fab1 gene and a mutant fae1 gene.

32. The method of claim 25 , wherein the plant material is a plant or a seed.

Assignees

• Shanklin John
• Nguyen Tam Huu
• Walsh Terence A
• Pidkowich Mark S
• Whittle Edward J
• Dow Agrosciences Llc
• Brookhaven Science Ass Llc

Inventors

• Shanklin John
• Nguyen Tam Huu
• Walsh Terence A
• Pidkowich Mark S
• Whittle Edward J

Classifications

• C12Y114/19001

  Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N9/0083 Primary

  Miscellaneous (1.14.99) · CPC title

• C12N15/8234

  Seed-specific, e.g. embryo, endosperm · CPC title