Targeted/immunomodulatory fusion proteins and methods for making same

Patent metadata

Field	Value
Publication number	US-9340617-B2
Application number	US-201414458674-A
Country	US
Kind code	B2
Filing date	Aug 13, 2014
Priority date	Apr 30, 2012
Publication date	May 17, 2016
Grant date	May 17, 2016

Abstract

The present invention relates generally to the field of generating fusion proteins to be used in cancer therapy, and more specifically, to nucleotide sequences encoding the fusion proteins, wherein the chimeric fusion proteins comprises at least one targeting moiety and at least one immunomodulatory moiety that counteracts the immune tolerance of cancer cells.

First claim

That which is claimed is:

1. A chimeric fusion protein comprising a targeting moiety to target a cancer cell and an immunomodulating moiety that counteracts immune tolerance, wherein the targeting moiety and the immunomodulating moiety are linked by an amino acid spacer selected from SEQ ID NO: 3 or SEQ ID NO: 11, wherein the immunomodulating moiety is TGF-βRII SEQ ID NO: 4, wherein the targeting moiety is Anti-HER2/neu having heavy chain SEQ ID NO: 1 and light chain SEQ ID NO: 2; wherein SEQ ID NO: 4 is attached via the amino acid spacer to the C-terminus of SEQ ID NO: 1 or SEQ ID NO: 2.

Assignees

• Biocon Ltd

Inventors

• Govindappa Nagaraj
• Sastry Kedarnath
• Soares Maria Melina

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• A61P37/04

  Immunostimulants · CPC title

• A61K38/179

  for growth factors; for growth regulators · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K2317/515

  Complete light chain, i.e. VL + CL · CPC title